Periostin, a stroma-associated protein, correlates with tumor invasiveness and progression in nasopharyngeal carcinoma
Periostin, a stroma-associated protein, correlates with tumor invasiveness and progression in nasopharyngeal carcinoma
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骨膜素是一种基质相关蛋白，与鼻咽癌的肿瘤侵袭性和进展相关
DOI：
10.1007/s10585-012-9465-5
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发表时间：
2012-12-01
影响因子：
4
通讯作者：
Chen, Zhuchu
中科院分区：
文献类型：
--
作者：
Li, Meixiang;Li, Cui;Chen, Zhuchu
近年来，肿瘤微环境在肿瘤的增殖、侵袭和转移中发挥着重要作用。为筛选与鼻咽癌（NPC）癌变相关的间质蛋白，采用激光捕获显微切割（LCM）和定量蛋白质组学方法，研究了NPC与正常鼻咽粘膜（NNM）间质蛋白表达的差异。在本研究中，与NNM基质相比，NPC基质中的periostin被鉴定为显著上调，并且通过Western印迹进一步证实了这一结果。免疫组化结果显示，Periostin在NPC间质及配对淋巴结转移灶（LNM）中的表达明显高于NNM间质。统计学分析显示，periostin的过度表达与鼻咽癌的临床分期（P< 0.001）、淋巴结转移（P< 0.001）及总生存率（P< 0.001）显著相关。考克斯回归分析显示periostin的过度表达是独立的预后因素。此外，利用periostin的异位表达检测其对鼻咽癌细胞体外侵袭能力的影响，结果表明periostin能够促进鼻咽癌细胞的侵袭能力。总之，骨膜蛋白表达与肿瘤分期、淋巴结转移和患者生存相关。Periostin是一种潜在的NPC分化和预后的生物标志物，在NPC的发生发展中可能起重要作用。
Recently, the tumor microenvironment is increasingly recognized as playing an important role in cancer proliferation, invasion, and metastasis. To screen stroma-associated proteins involved in nasopharyngeal carcinoma (NPC) carcinogenesis, laser capture microdissection (LCM) and quantitative proteomic analysis were employed to assess different protein expression of the stroma between NPC and normal nasopharyngeal mucosa (NNM). In this study, periostin was identified to be significantly up-regulated in NPC stroma compared with NNM stroma and the result was further confirmed by Western blotting. Immunohistochemistry showed that over-expression of periostin was frequently observed in the stroma of NPC and matched lymph node metastases (LNM) compared with the stroma of NNM. Statistical analysis showed over-expression of periostin was significantly associated with advanced clinical stage (P< 0.001) and lymph node metastasis (P< 0.001) and decreased overall survival (P< 0.001) in NPC. Cox regression analysis indicated over-expression of periostin was an independent prognostic factor. Furthermore, ectopic expression of periostin was used to examine its effect on invasiveness of NPC cell in vitro and the result showed that periostin was able to promote invasiveness of NPC cell. In conclusion, periostin expression is correlated with tumor stage, lymph node metastasis, and patient survival. Periostin is a potential biomarker for the differentiation and prognosis of NPC, and it might play an important role in the progression of NPC.