Molecular analysis of six dog leukocyte antigen class I sequences including three complete genes, two truncated genes and one full-length processed gene
Molecular analysis of six dog leukocyte antigen class I sequences including three complete genes, two truncated genes and one full-length processed gene
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DOI：
10.1111/j.1399-0039.1997.tb02783.x
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发表时间：
1997-05-01
期刊：
影响因子：
--
通讯作者：
Storb, R
中科院分区：
文献类型：
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作者：
Burnett, RC;DeRose, SA;Storb, R
我们分离了6个不同的犬白细胞抗原（DLA） I类序列。另外一个功能性的非经典I类基因，DLA-79，先前已被鉴定。这使得分离的犬类I类序列达到7个。这7个位点几乎占了南方印迹法在基因组DNA中检测到的所有I类序列。狗的I类基因家族大约有7个成员，比人类或老鼠的要简单得多。本文描述的六个序列中有三个包含完整的I类基因。这些基因具有典型的外显子和内含子排列，其预测的蛋白序列具有预期表达的I类分子的特征。两个序列包含截断的基因。这些基因，只有部分I类同源性和破坏性突变，显然是无功能的。剩余的位点包含一个全长加工基因，这在特征的I类位点中是独一无二的。序列比较是为了检验家族成员之间的进化关系。两个序列，DLA-64（完整）和-53（截断），似乎是嵌合体，可能是由基因座间重组形成的。利用逆转录聚合酶链反应，在犬外周血白细胞中分别鉴定了三个完整基因的mRNA。DLA-79特异性转录本先前已确定。因此，四个完整的犬类I类基因中的每一个都是在体内转录的。
We have isolated six distinct dog leukocyte antigen (DLA) class I sequences. An additional functional nonclassical class I gene, DLA-79, was characterized previously. This brings the number of isolated canine class I sequences to seven. These seven loci account for nearly all class I sequences detected in genomic DNA by Southern blotting. With approximately seven members, the class I gene family in dog is considerably less complex than in either human or mouse. Three of the six sequences described in this article contain complete class I genes. These genes have the typical arrangement of exons and introns, and their predicted protein sequences have the features expected of expressed class I molecules. Two sequences contain truncated genes. These genes, having only partial class I homology and disruptive mutations, are clearly nonfunctional. The remaining locus contains a full-length processed gene which is unique among characterized class I loci. Sequence comparisons were performed to examine evolutionary relationships among the family members. Two sequences, DLA-64 (complete) and -53 (truncated), appear to be chimeras presumably formed by interlocus recombination. Using the reverse transcription-polymerase chain reaction, mRNA originating from each of the three complete genes was identified in canine peripheral blood leukocytes. DLA-79 specific transcripts were identified previously. Thus, each of the four complete canine class I genes is transcribed in vivo.