IGFBP1 and Follistatin-like 3 genes are significantly up-regulated in expression profiles of the IUGR placenta
IGFBP1 and Follistatin-like 3 genes are significantly up-regulated in expression profiles of the IUGR placenta
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DOI：
10.1016/j.placenta.2004.12.007
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发表时间：
2006-02-01
期刊：
影响因子：
3.8
通讯作者：
Tanaka, T
中科院分区：
文献类型：
--
作者：
Okamoto, A;Endo, H;Tanaka, T
迄今为止，对宫内生长受限（IUGR）的临床病理特征还不清楚，也没有有效的治疗方法。这是第一个使用微阵列分析来鉴定IUGR胎盘中差异表达基因的研究。共9121个基因的表达谱进行了研究，使用mRNA从适当的胎龄（阿加）胎盘和IUGR胎盘不和谐的胎儿发育不良双胞胎的cDNA微阵列分析。在IUGR胎盘中检测到IGFBP 1和卵泡抑素样3基因的上调，平衡差异度分别为20.7 +/- 1.3和13.1 +/- 2.1，采用RT-PCR方法检测IGFBP 1和Follistatin-like 3基因在4例IUGR和4例阿加胎盘组织中的表达。PCR法与我们在不一致绒毛膜双胎胎盘中的数据一致，四个IUGR胎盘中的三个显示IGFBP 1上调，并且所有四个IUGR胎盘均显示上调。与阿加胎盘相比，我们的研究结果表明IGFBP 1和卵泡抑素样3在胎盘中的IUGR中高度上调。IGFBP 1和卵泡抑素样3是已知的胎儿生长和分化的关键调节因子。与这些基因相关的通路可能在IUGR的发病机制中起重要作用。(c)2005爱思唯尔有限公司保留所有权利。
To date, the clinicopathological features of intrauterine growth restriction (IUGR) are not clearly understood, and no effective therapy has been established for IUGR. This is the first study that uses microarray analysis to identify differentially expressed genes in the IUGR placenta. The expression profiles of a total of 9121 genes were examined by cDNA microarray analysis, using mRNA from an appropriate gestational age (AGA) placenta and an IUGR placenta from discordant dichorionic twins. Upregulation of the IGFBP1 and Follistatin-like 3 genes was detected in the IUGR placenta, with a balanced differential degree of 20.7 +/- 1.3 and 13.1 +/- 2.1, respectively, while the balanced differential degrees of other genes were 2.6 or less. The expressions of the IGFBP1 and Follistatin-like 3 genes in four single IUGR and four AGA placentas were also examined by RT-PCR. Consistent with our data in discordant chorionic twin placentas, three of four IUGR placentas showed up-regulation of the IGFBP1 and all four IUGR placentas showed upregulation. of Follistatin-like 3 genes when compared to the AGA placentas. Our results suggest that IGFBP1 and Follistatin-like 3 are highly up-regulated in IUGR in the placenta. IGFBP1 and Follistatin-like 3 are known critical regulators of fetal growth and differentiation. Pathways associated with these genes might be important for the pathogenesis of IUGR. (c) 2005 Elsevier Ltd. All rights reserved.