A MACROPHAGE MESSENGER-RNA SELECTIVELY INDUCED BY GAMMA-INTERFERON ENCODES A MEMBER OF THE PLATELET FACTOR-IV FAMILY OF CYTOKINES
A MACROPHAGE MESSENGER-RNA SELECTIVELY INDUCED BY GAMMA-INTERFERON ENCODES A MEMBER OF THE PLATELET FACTOR-IV FAMILY OF CYTOKINES
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DOI：
10.1073/pnas.87.14.5238
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发表时间：
1990-07-01
影响因子：
11.1
通讯作者：
FARBER, JM
中科院分区：
文献类型：
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作者：
FARBER, JM
为了鉴定在活化的巨噬细胞中合成的新介质，我们从小鼠巨噬细胞系RAW 264.7的培养物中制备了一个cDNA文库，该细胞系经富含淋巴因子的条件培养基处理，培养基来自有丝分裂原刺激的小鼠脾细胞。差异斑块杂交鉴定出一种cDNA，命名为m119，检测到1.6千碱基的mRNA，该mRNA在. γ的作用下积累。-干扰素（IFN-. γ），但对其他巨噬细胞激活剂（包括IFN-. α）没有反应。干扰素度量。和脂多糖。mRNA编码的预测蛋白Mr 14461含有21个氨基酸的信号肽。预测蛋白的初级结构表明，它是最近描述的与血小板因子4相关的细胞因子家族的成员，包括Gro/黑色素瘤生长刺激活性和中性粒细胞激活肽/白细胞介素8。IFN- γ对m119mrna的选择性诱导。表明预测的m119蛋白介导了IFN- γ调节的巨噬细胞活性。m119蛋白可能是一种细胞因子，影响参与免疫和炎症反应的细胞的生长、运动或激活状态。有人建议将编码该蛋白的基因命名为mig，即γ干扰素诱导的单因子。
In order to identify novel mediators synthesized in activated macrophages, a cDNA library was prepared from cultures of the mouse macrophage cell line RAW 264.7 that had been treated with lymphokine-rich conditioned medium from mitogen-stimulated mouse spleen cells. Differential plaque hybridization identified a cDNA, designated m119, that detected a 1.6-kilobase mRNA that accumulated in response to .gamma.-interferon (IFN-.gamma.) but not in response to other macrophage activators, including IFN-.alpha., IFN-.beta., and lipopolysaccharide. The mRNA encoded a predicted protein Mr 14,461 containing a 21-amino acid signal peptide. The primary structure of the predicted protein indicated that it is a member of a recently described family of cytokines related to platelet factor 4, including Gro/melanoma growth stimulatory activity and neutrophil-activating peptide/interleukin 8. The selective induction of the m119 mRNA by IFN-.gamma. suggests that the predicted m119 protein mediates a macrophage activity regulated by IFN-.gamma.. The m119 protein may be a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory responses. It is proposed that the gene encoding this protein be called mig, for monokine induced by gamma interferon.