Distribution of F-actin and Microtubules in Pollen and Pollen Tube of Lilium davidii
Distribution of F-actin and Microtubules in Pollen and Pollen Tube of Lilium davidii
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发表时间：
1998-10
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通讯作者：
Li Yan;Yan Long-fei;Xu Shi-xiong
中科院分区：
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作者：
Li Yan;Yan Long-fei;Xu Shi-xiong
肌动蛋白和微管是花粉管的重要组成部分，在花粉管的胞质流动中起着重要的作用。观察了兰州百合（LiliumdavidiiDuch）花粉管中肌动蛋白和微管的分布。应用免疫荧光技术和共聚焦激光扫描显微镜技术，获得了一些新的结果。1.化学固定法能较好地保存F actin在花粉管中的分布，因此可以通过化学固定法和荧光标记法研究F actin与微管的关系。2.在花粉管端部，F肌动蛋白束缺失，微管丰富，并形成网状结构。作者发现，在花粉管顶端，微管末端与质膜紧密相连。3.兰州百合花粉管中只有少数肌动蛋白束与微管共存。这一结果为F肌动蛋白与微管在花粉管中的作用及其相互关系提供了新的证据。
F actin and microtubules are important components of pollen tube, which have very important function in cytoplasm streaming of pollen tube. The authors observed the distribution of F actin and microtubules in the pollen tube of Lilium davidii Duch. by immunofluorescence technique and confocol laser scanning microscopy, through which some new results were obtained. 1. Chemical fixation could preserve F actin well in pollen tube, so the relation between F actin and microtubules could be studied by the methods of chemical fixation and fluorescence labelling in pollen tube. 2. F actin bundles were absent near the pollen tube tip, while microtubules were abundant and web formed in the pollen tube tip. The authors found that the terminal of microtubules was closely associated with the plasma membrane in the pollen tube tip. 3. Only a few F actin bundles co exist with the microtubules in the pollen tube of Lilium davidii . The results provided new evidence for the function and relationship between F actin and microtubules in the pollen tube.