Phosphate Transporter PstSCAB of Campylobacter jejuni Is a Critical Determinant of Lactate-Dependent Growth and Colonization in Chickens.
Phosphate Transporter PstSCAB of Campylobacter jejuni Is a Critical Determinant of Lactate-Dependent Growth and Colonization in Chickens.
复制标题
空肠弯曲菌的磷酸转运蛋白 PstSCAB 是鸡中乳酸依赖性生长和定植的关键决定因素。
DOI：
10.1128/jb.00716-19
复制
发表时间：
2020
影响因子：
3.2
通讯作者：
DiRita,VictorJ
中科院分区：
文献类型：
--
作者：
Sinha,Ritam;LeVeque,RhiannonM;Bowlin,MarvinQ;Gray,MichaelJ;DiRita,VictorJ
空肠弯曲杆菌在世界范围内引起急性胃肠炎，主要通过家禽传播，它通常是肠道微生物群的一个重要成员。先前的转录组测序（RNA-Seq）实验表明，来自编码C.当细菌在鸡体内生长时，空肠是最丰富的。在来自人类感染研究的RNA-Seq实验中也鉴定了升高水平的pstSCABmRNA。在这项研究中，我们探讨了PstSCAB的生物学和C。空肠。我们的结果表明，缺乏PstSCAB的细胞在稳定期、营养限制性培养基以及反映鸡中的渗透条件下存活较差。突变体细胞中的多磷酸盐水平在稳定期升高，与多磷酸盐代谢基因表达的改变一致。该突变株高度减毒的新孵化的小鸡殖民，在几个数量级低于野生型水平的细菌水平。突变体和野生型在复合培养基中生长相似，但pstS：：kan突变体在补充l-乳酸的基本培养基中表现出明显的生长缺陷，l-乳酸被假定为体内碳源。乳酸生长不良与先前证明对定殖鸡重要的产乙酸途径基因的表达减少相关。因此，磷酸盐转运系统对C.重要的是空肠弯曲杆菌每年引起数百万人的胃肠道感染，家禽是主要的感染源。由于C.空肠，有必要确定控制这种病原体的替代方法。编码高亲和力磷酸盐转运蛋白PstSCAB的基因在C.鸡和人的空肠。在这项研究中，我们解决的作用，PstSCAB对鸡的殖民和其他C。空肠表型PstSCAB在鸡中的定殖、在不同应激反应下的代谢和存活以及在鸡中的潜在生长底物乳酸盐上的生长期间是必需的。我们的研究强调，PstSCAB可能是一个有效的目标，以制定机制，控制细菌负担在鸡和人。
Campylobacter jejuni causes acute gastroenteritis worldwide and is transmitted primarily through poultry, in which it is often a commensal member of the intestinal microbiota. Previous transcriptome sequencing (RNA-Seq) experiment showed that transcripts from an operon encoding a high-affinity phosphate transporter (PstSCAB) of C. jejuni were among the most abundant when the bacterium was grown in chickens. Elevated levels of thepstSCABmRNA were also identified in an RNA-Seq experiment from human infection studies. In this study, we explore the role of PstSCAB in the biology and colonization potential of C. jejuni. Our results demonstrate that cells lacking PstSCAB survive poorly in stationary phase, in nutrient-limiting media, and under osmotic conditions reflective of those in the chicken. Polyphosphate levels in the mutant cells were elevated at stationary phase, consistent with alterations in expression of polyphosphate metabolism genes. The mutant strain was highly attenuated for colonization of newly hatched chicks, with levels of bacteria at several orders of magnitude below wild-type levels. Mutant and wild type grew similarly in complex media, but thepstS::kanmutant exhibited a significant growth defect in minimal medium supplemented withl-lactate, postulated as a carbon sourcein vivo. Poor growth in lactate correlated with diminished expression of acetogenesis pathway genes previously demonstrated as important for colonizing chickens. The phosphate transport system is thus essential for diverse aspects of C. jejuni physiology andin vivofitness and survival.IMPORTANCECampylobacter jejuni causes millions of human gastrointestinal infections annually, with poultry a major source of infection. Due to the emergence of multidrug resistance in C. jejuni, there is need to identify alternative ways to control this pathogen. Genes encoding the high-affinity phosphate transporter PstSCAB are highly expressed by C. jejuni in chickens and humans. In this study, we address the role of PstSCAB on chicken colonization and other C. jejuni phenotypes. PstSCAB is required for colonization in chicken, metabolism and survival under different stress responses, and during growth on lactate, a potential growth substrate in chickens. Our study highlights that PstSCAB may be an effective target to develop mechanisms for controlling bacterial burden in both chicken and human.