Amino acid sequence of rat liver thiol proteinase inhibitor.
Amino acid sequence of rat liver thiol proteinase inhibitor.
复制标题
大鼠肝硫醇蛋白酶抑制剂的氨基酸序列。
DOI：
10.1016/s0006-291x(83)80020-5
复制
发表时间：
1983
影响因子：
3.1
通讯作者：
K. Titani
中科院分区：
文献类型：
--
作者：
K. Takio;E. Kominami;N. Wakamatsu;N. Katunuma;K. Titani
给出了大鼠肝巯基蛋白酶抑制剂的完整氨基酸序列。其独特的98个残基的序列主要来自于分析有限的蛋白水解产物的分离过程中产生的蛋白质与活性木瓜蛋白酶亲和柱和裂解产生的片段在乙酰基键。用连四硫酸盐灭活的无花果蛋白酶柱制备的完整蛋白具有独特的氨基末端序列（Ac-Met-Met-Cys-）。该抑制剂与任何已知结构的蛋白酶抑制剂没有序列同源性。
The complete amino acid sequence of rat liver thiol proteinase inhibitor is presented. Its unique 98 residue sequence was derived mostly from analyses of a limited proteolysis product generated during the isolation of the protein with an active papain affinity column and of fragments produced by cleavage at arginyl bonds. The intact protein prepared with a tetrathionate inactivated ficin column has a unique amino-terminal sequence (Ac-Met-Met-Cys-). The inhibitor has no sequence homology with any proteinase inhibitor of known structure.