A field study of pollen-mediated gene flow from Mediterranean GM rice to conventional rice and the red rice weed
A field study of pollen-mediated gene flow from Mediterranean GM rice to conventional rice and the red rice weed
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DOI：
10.1023/b:molb.0000012285.39859.9d
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发表时间：
2004-01-01
影响因子：
3.1
通讯作者：
Melé, E
中科院分区：
文献类型：
--
作者：
Messeguer, J;Marfà, V;Melé, E
本研究的目的是评估以gusA和bar基因分别编码β -葡糖苷酸酶和磷脂酰丙氨酸乙酰转移酶为标记的转基因水稻品系花粉介导的基因流到西班牙粳稻品种Senia的红稻杂草和常规水稻的频率。采用圆形田间试验设计，研究了风对红稻和普通水稻受粉植株授粉频率的影响。在不同风向下，红稻和普通稻的抗除草剂基因流频率分别为0.036 +/- 0.006%和0.086 +/- 0.007。然而，对于红米和常规水稻，观察到明显的不对称分布，授粉频率有利于放置在当地盛行风下的植株。Southern分析证实了半合子状态和转基因在存活的GUS阳性植物后代中的起源。常规水稻在1、2、5和10 m距离上的基因流随距离的增加而明显减少，在盛行风向下不明显。讨论了这些发现对遏制转基因水稻向红稻杂草的基因流动的影响。准确测定花期和授粉日的当地风况，对于确定合适的隔离距离至关重要。
The objective of this study was to assess the frequency of pollen-mediated gene flow from a transgenic rice line, harbouring the gusA and the bar genes encoding respectively, beta-glucuronidase and phosphinothricin acetyl transferase as markers, to the red rice weed and conventional rice in the Spanish japonica cultivar Senia. A circular field trial design was set up to investigate the influence of the wind on the frequency of pollination of red rice and conventional rice recipient plants with the transgenic pollen. Frequencies of gene flow based on detection of herbicide resistant, GUS positive seedlings among seed progenies of recipient plants averaged over all wind directions were 0.036 +/- 0.006% and 0.086 +/- 0.007 for red rice and conventional rice, respectively. However, for both red rice and conventional rice, a clear asymmetric distribution was observed with pollination frequency favoured in plants placed under the local prevailing winds. Southern analyses confirmed the hemizygous status and the origin of the transgenes in progenies of surviving, GUS positive plants. Gene flow detected in conventional rice planted at 1, 2, 5 and 10 m distance revealed a clear decrease with increasing distance which was less dramatic under the prevailing wind direction. Consequences of these findings for containment of gene flow from transgenic rice crops to the red rice weed are discussed. The precise determination of the local wind conditions at flowering time and pollination day time appear to be of primary importance for setting up suitable isolation distances.