The efficacy of the heat killing of Mycobacterium tuberculosis
The efficacy of the heat killing of Mycobacterium tuberculosis
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DOI：
10.1136/jcp.55.10.778
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发表时间：
2002-10-01
影响因子：
3.4
通讯作者：
Forbes, KJ
中科院分区：
文献类型：
--
作者：
Doig, C;Seagar, AL;Forbes, KJ
人们担心，目前的结核分枝杆菌热灭活程序可能是不够的。这对进行分子研究如IS6110限制性片段长度多态性分型的实验室工作人员提出了严重的安全问题。本文证实了货车Embden等人的方案，在本实验室常规进行，是安全和有效的结核分枝杆菌热灭活。该程序包括将含有一个满环生长物的悬浮液的试管完全浸入80 ℃水浴中20分钟。本次调查共纳入74株分离株。尽管长时间孵育20周，但热灭活的结核分枝杆菌悬浮液均未产生可见菌落或从液体培养物中产生阳性生长信号。该方法不影响后续分子研究的DNA完整性。
There is concern that current procedures for the heat inactivation of Mycobacterium tuberculosis may not be adequate. This raises serious safety issues for laboratory staff performing molecular investigations such as IS61 10 restriction fragment length polymorphism typing. This paper confirms that the protocol of van Embden et al, as performed routinely in this laboratory, is safe and effective for the heat inactivation of M tuberculosis. This procedure involves complete immersion of a tube containing a suspension of one loopfull of growth in a water bath at 80degreesC for 20 minutes. Seventy four isolates were included in this investigation. Despite prolonged incubation for 20 weeks, none of the heat killed M tuberculosis suspensions produced visible colonies or gave a positive growth signal from liquid culture. This method did not affect the integrity of the DNA for subsequent molecular investigations.