The application of an enzyme-linked immunosorbent assay or an immunofluorescent assay test leads to different estimates of seroprevalence of Coxiella burnetii in the population
The application of an enzyme-linked immunosorbent assay or an immunofluorescent assay test leads to different estimates of seroprevalence of Coxiella burnetii in the population
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DOI：
10.1017/s0950268811000021
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发表时间：
2012-01-01
影响因子：
4.2
通讯作者：
Schneeberger, P. M.
中科院分区：
文献类型：
--
作者：
Blaauw, G. J.;Notermans, D. W.;Schneeberger, P. M.
Q热的诊断和流行病学研究依赖于血清学。所采用的主要方法有酶联免疫吸附试验（ELISA）和免疫荧光试验（IFAT）。我们发现，两种商业检测代表两种方法，两种不同的截止滴度可以导致显着差异的诊断和血清阳性率估计。这反过来又强调了需要一个标准化的黄金方法来比较各种测定;无论这个标准是“内部”或商业获得。
The diagnosis and epidemiological studies of Q fever depend on serology. Among the main methods employed are the enzyme-linked immunosorbent assay (ELISA) and the immunofluorescent assay test (IFAT). We show that two commercial assays representing the two methods with two different cut-off titres can lead to significant differences in diagnostic and seroprevalence estimates. This in turn emphasizes the need for a standardized gold method to compare the various assays; whether this standard is 'in-house' or commercially obtained.