Self-made frits for nanoscale columns in proteomics
Self-made frits for nanoscale columns in proteomics
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DOI：
10.1002/pmic.200402010
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发表时间：
2005-10-01
期刊：
影响因子：
3.4
通讯作者：
Rappsilber, J
中科院分区：
文献类型：
--
作者：
Maiolica, A;Borsotti, D;Rappsilber, J
我们在这里报告的生产自制的纳米柱熔块。玻璃料引入了较小的死体积，并且可以以极其简单和低成本的程序放置在具有宽范围直径（20-250 μ m测试）的毛细管中。所获得的列似乎是可比的接受：2005年1月11日，以“无玻璃料”列与接近理想的色谱特性。我们期望这种玻璃料将用于在MALDI板上的梯度点样，而且在特殊的ESI设置不允许“无玻璃料”解决方案的情况下也有用。
We report here the production of self-made frits for nano-columns. The frits introduce a minor Received: November 24, 2004 dead volume and can be placed in capillaries with a wide range of diameters (20-250 Pm tested) Revised: December 21, 2004 in an extremely simple and low-cost procedure. The obtained columns appear to be comparable Accepted: January 11, 2005 to "no-frit" columns with near-ideal chromatographic characteristics. We expect that this frit will be useful for the spotting of gradients onto MALDI plates but also where special ESI set-ups do not allow for "no-frit" solutions.