Poly(vinyl alcohol)/poly(acrylic acid) hydrogel coatings for improving electrode-neural tissue interface
Poly(vinyl alcohol)/poly(acrylic acid) hydrogel coatings for improving electrode-neural tissue interface
复制标题
DOI：
10.1016/j.biomaterials.2009.04.030
复制
发表时间：
2009-09-01
期刊：
影响因子：
14
通讯作者：
Duan, Yanwen Y.
中科院分区：
文献类型：
--
作者：
Lu, Yi;Wang, Dingfang;Duan, Yanwen Y.
长期植入过程中组织反应引起的性能不一致是阻碍神经假体应用的主要问题。本研究探索了一种改善电极-神经组织界面的新方法。合成了水凝胶聚乙烯醇/聚丙烯酸互穿聚合物网络（PVA/PAA IPNs），并采用等离子体预处理技术制备了聚二甲基硅氧烷（PDMS）神经电极涂层。涂层氧化铱微电极的电化学阻抗和最大电荷注入（Q1）极限的变化可以忽略不计。涂覆后，PDMS上的蛋白质吸附减少了约85%。在神经生长因子（NGF）的存在下，大鼠嗜铬细胞瘤（PC 12）细胞的神经突起的延伸明显大于PVA/PAA IPN膜上的PDMS基板。此外，通过在大鼠皮层中植入6周来研究涂覆有PVA/PAA IPN膜的PDMS植入物的组织反应，发现接受涂层植入物的动物（n = 8）中的胶质细胞酸性蛋白（GFAP）免疫反应性显著降低，（p < 0.05）与无涂层种植体（n = 7）相比，沿从外裙部到种植体界面的150 μ m的整个距离沿着。通过扫描电子显微镜（SEM）证实，涂层膜保留在植入物表面。这些都表明，水凝胶涂层是可行的，有利于神经电极的应用。(C)2009爱思唯尔有限公司保留所有权利。
A major problem which hinders the applications of neural prostheses is the inconsistent performance caused by tissue responses during long-term implantation. The study investigated a new approach for improving the electrode-neural tissue interface. Hydrogel poly(vinyl alcohol)/poly(acrylic acid) interpenetrating polymer networks (PVA/PAA IPNs) were synthesized and tailored as coatings for poly(dimethylsiloxane) (PDMS) based neural electrodes with the aid of plasma pretreatment. Changes in the electrochemical impedance and maximum charge injection (Q 1) limits of the coated iridium oxide microelectrodes were negligible. Protein adsorption on PDMS was reduced by similar to 85% after coating. In the presence of nerve growth factor (NGF), neurite extension of rat pheochromocytoma (PC12) cells was clearly greater on PVA/PAA IPN films than on PDMS substrates. Furthermore, the tissue responses of PDMS implants coated with PVA/PAA IPN films were studied by 6-week implantation in the cortex of rats, which found that the glial fibrillary acidic protein (GFAP) immunoreactivity in animals (n = 8) receiving coated implants was significantly lower (p < 0.05) compared to that of uncoated implants (n = 7) along the entire distance of 150 mu m from the outer skirt to the implant interface. The coated film remained on the surface of the explanted implants, confirmed by scanning electron microscopy (SEM). All of these suggest the hydrogel coating is feasible and favorable to neural electrode applications. (C) 2009 Elsevier Ltd. All rights reserved.