Borrelia burgdorferi locus BB0795 encodes a BamA orthologue required for growth and efficient localization of outer membrane proteins.
Borrelia burgdorferi locus BB0795 encodes a BamA orthologue required for growth and efficient localization of outer membrane proteins.
复制标题
DOI：
10.1111/j.1365-2958.2009.07015.x
复制
发表时间：
2010-03
影响因子：
3.6
通讯作者：
Akins DR
中科院分区：
文献类型：
--
作者：
Lenhart TR;Akins DR
致病性伯氏疏螺旋体的外膜除含有大量的外表面脂蛋白外，还含有完整的β-Barrel外膜蛋白。在伯氏假单胞菌中发现的OMP很少，OMP组装和OM定位所需的蛋白质机制目前尚不清楚。最近在革兰氏阴性细菌中鉴定了必要的OM BAMA蛋白，这些蛋白是OMβ桶装配机的核心组件，是正确定位和插入细菌OMP所必需的。在本研究中，我们鉴定了一个由开放阅读框架bb0795编码的假定的Burgdorferi Bama同源基因。结构模型预测和细胞定位数据表明，Burgdorferi BB0795蛋白含有一个N端的周质结构域和一个C端的表面暴露的β-Barrel结构域。此外，对IPTG可调节的bb0795突变体的分析表明，BB0795是伯氏杆菌生长所必需的。此外，BB0795的耗尽会导致伯氏杆菌OM中可检测到的OMP数量减少。有趣的是，在突变的OM中也观察到暴露在表面的脂蛋白水平的下降。总的来说，我们的结构、细胞定位和功能数据与其他BAMA蛋白的特征一致，表明BB0795是B.burgdorferi Bama的同源物。
The outer membrane (OM) of the pathogenic diderm spirochete, Borrelia burgdorferi, contains integral β-barrel outer membrane proteins (OMPs) in addition to its numerous outer surface lipoproteins. Very few OMPs have been identified in B. burgdorferi, and the protein machinery required for OMP assembly and OM localization is currently unknown. Essential OM BamA proteins have recently been characterized in Gram-negative bacteria that are central components of an OM β-barrel assembly machine and are required for proper localization and insertion of bacterial OMPs. In the present study, we characterized a putative B. burgdorferi BamA orthologue encoded by open reading frame bb0795. Structural model predictions and cellular localization data indicate that the B. burgdorferi BB0795 protein contains an N-terminal periplasmic domain and a C-terminal, surface-exposed β-barrel domain. Additionally, assays with an IPTG-regulatable bb0795 mutant revealed that BB0795 is required for B. burgdorferi growth. Furthermore, depletion of BB0795 results in decreased amounts of detectable OMPs in the B. burgdorferi OM. Interestingly, a decrease in the levels of surface-exposed lipoproteins was also observed in the mutant OMs. Collectively, our structural, cellular localization and functional data are consistent with the characteristics of other BamA proteins, indicating that BB0795 is a B. burgdorferi BamA orthologue.
登录
查看更多内容
影响因子：
3.6
作者：
Eggers, CH;Caimano, MJ;Radolf, JD
通讯作者：
Radolf, JD
影响因子：
4.8
作者：
Doerrler, WT;Raetz, CRH
通讯作者：
Raetz, CRH
影响因子：
14.9
作者：
Bagos, PG;Liakopoulos, TD;Hamodrakas, SJ
通讯作者：
Hamodrakas, SJ
影响因子：
3.2
作者：
BELISLE, JT;BRANDT, ME;NORGARD, MV
通讯作者：
NORGARD, MV
影响因子：
3.1
作者：
Brooks, CS;Vuppala, SR;Akins, DR
通讯作者：
Akins, DR