IMMUNOCYTOLOGICAL RESPONSES IN PORCINE PROLIFERATIVE ENTEROPATHIES
IMMUNOCYTOLOGICAL RESPONSES IN PORCINE PROLIFERATIVE ENTEROPATHIES
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DOI：
10.1128/iai.60.10.4184-4191.1992
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发表时间：
1992-10-01
影响因子：
3.1
通讯作者：
LAWSON, GHK
中科院分区：
文献类型：
--
作者：
MCORIST, S;MACINTYRE, N;LAWSON, GHK
使用针对猪免疫系统元件的抗体，通过免疫细胞化学检查自然受到两种主要形式的增殖性肠病（即肠腺瘤病或出血性肠病）影响的猪的回肠、结肠和肠系膜淋巴结对感染的免疫细胞学反应。在这两种形式中，未成熟肠细胞的粘膜增殖，缺乏大量主要组织相容性复合物 II 类表达，并且与细胞内弯曲杆菌样生物相关的受影响肠细胞的顶端细胞质中免疫球蛋白 A (IgA) 显着积累。在肠腺瘤病中，肠固有层仅有轻度CD8+和CD25+T细胞浸润。在出血性肠病中，固有层有中度的 CD8+ 和 CD25+ T 细胞以及 IgM+ B 细胞浸润。在大鼠和人类中，绒毛肠上皮细胞被认为充当抗原呈递细胞，其表面存在主要组织相容性复合物 II 类分子，能够启动 T 细胞反应（特别是 CD8+ T 细胞）以响应细菌抗原。因此，细胞内弯曲杆菌样生物选择未成熟的隐窝细胞进行进入和增殖可能代表了与局部免疫调节和增强细菌存活相关的显着的微生物适应。受影响的肠细胞内 IgA 的积累可能代表细胞处理非特异性 IgA 的能力降低或特异性 IgA 的积累。
The ileum, colon, and mesenteric lymph nodes of pigs naturally affected by either of the two major forms of proliferative enteropathy, namely, intestinal adenomatosis or hemorrhagic enteropathy, were examined for immunocytological responses to infection by immunocytochemistry, using antibodies directed against elements of the porcine immune system. In both forms, there was mucosal proliferation of immature enterocytes which lacked substantial major histocompatibility complex class II expression and a marked accumulation of immunoglobulin A (IgA) at the apical cytoplasm of affected enterocytes in association with intracellular Campylobacter-like organisms. In intestinal adenomatosis, there was only a mild infiltration of CD8+ and CD25+ T cells in the intestinal lamina propria. In hemorrhagic enteropathy, there was a moderate infiltration of CD8+ and CD25+ T cells and IgM+ B cells in the lamina propria. In rats and humans, villous enterocytes are thought to act as antigen-presenting cells, with major histocompatibility complex class II molecules present on their surface, capable of initiating a T-cell response (particularly of CD8+ T cells) in response to bacterial antigens. Therefore, the selection of immature crypt cells by the intracellular Campylobacter-like organisms for entry and multiplication may represent a remarkable microbial adaptation associated with local immunomodulation and enhanced bacterial survival. The accumulation of IgA within affected enterocytes may represent a reduced capability of the cells to process nonspecific IgA or an accumulation of specific IgA.