ESTRADIOL-17BETA AND PROLACTIN LEVELS IN RAT PERIPHERAL PLASMA
ESTRADIOL-17BETA AND PROLACTIN LEVELS IN RAT PERIPHERAL PLASMA
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DOI：
10.1038/bjc.1975.147
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发表时间：
1975-01-01
影响因子：
8.8
通讯作者：
KILLEN, E
中科院分区：
文献类型：
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作者：
HAWKINS, RA;FREEDMAN, B;KILLEN, E
研究了测定雌二醇-17 β和催乳素的放射免疫测定方法的可靠性，并将其应用于测定雄性和雌性大鼠血浆中的激素浓度。在所有样本中均可检测到催乳素（大于7 ng RP-1 ng/ml），但在卵巢切除的女性中，雌二醇-17 β的浓度低于该方法的灵敏度（大于0-10 ng/100 ml）。血浆雌二醇-17 β浓度从早孕期到发情期逐渐上升，在发情期降至几乎检测不到的水平。血浆催乳素浓度变化很大，即使在同一发情周期的大鼠，但在发情的下午，催乳素浓度最小，而在发情的下午，催乳素的分泌激增。催乳素浓度除受发情周期阶段影响外，还受采血方式、溶血程度、血清或血浆选择的影响。在同一血浆样品中，催乳素浓度与雌二醇-17 β浓度无相关性。
Radioimmunoassay methods for the determination of oestradiol-17 beta and prolactin have been examined for their reliability and applied to the measurement of hormone concentrations in the plasmas from male and female rats. Prolactin was detectable in all samples (greater than 7 ng RP-1 ng/ml) but the concentration of oestradiol-17 beta was below the sensitivity of the method (greater than 0-10 ng/100 ml) in ovariectomized females. Plasma oestradiol-17 beta concentration rose gradually from metoestrus to proestrus and fell to barely detectable levels in oestrus. Plasma prolactin concentration was very variable even for rats in the same stage of the oestrous cycle but values were minimal in the afternoon of diestrus and a surge in secretion occurred in the afternoon of proestrus. In addition to the stage of the oestrous cycle, the prolactin concentration was influenced by mode of blood collection, degree of haemolysis and choice of serum or plasma. There was no correlation between the concentration of prolactin and that of oestradiol-17 beta in the same sample of plasma.