Thyroid hormone regulates hippocampal neurogenesis in the adult rat brain
Thyroid hormone regulates hippocampal neurogenesis in the adult rat brain
复制标题
DOI：
10.1016/j.mcn.2005.03.010
复制
发表时间：
2005-07-01
影响因子：
3.5
通讯作者：
Vaidya, VA
中科院分区：
文献类型：
--
作者：
Desouza, LA;Ladiwala, U;Vaidya, VA
我们研究了甲状腺激素对成年海马神经发生的影响，包括齿状颗粒祖细胞的增殖、存活和分化。使用溴脱氧尿苷（BrdU），我们证明，成人发病甲减显着降低海马神经发生。这种下降主要是BrdU阳性细胞的存活率和神经元分化显著降低的结果。恢复甲状腺功能正常状态可以挽救海马前体细胞的存活率和神经元分化。成年发病的甲状腺功能亢进症不影响海马神经发生，这表明甲状腺激素的作用可能是最佳的甲状腺功能正常的水平。我们在体内和体外的结果表明，成年海马祖细胞表达甲状腺受体亚型。体外研究表明，成年海马祖细胞表现出增强的增殖，存活和胶质细胞分化的甲状腺激素的反应。这些结果支持甲状腺激素在调节成人海马神经发生中的作用，并提高了神经发生改变可能导致与成人发病甲状腺功能减退症相关的认知和行为缺陷的可能性。(c)2005年爱思唯尔公司All rights reserved.
We have examined the influence of thyroid hormone on adult hippocampal neurogenesis, which encompasses the proliferation, survival and differentiation of dentate granule cell progenitors. Using bromodeoxyuridine (BrdU), we demonstrate that adult-onset hypothyroidism significantly decreases hippocampal neurogenesis. This decline is predominantly the consequence of a significant decrease in the survival and neuronal differentiation of BrdU-positive cells. Both the decreased survival and neuronal differentiation of hippocampal progenitors could be rescued by restored euthyroid status. Adult-onset hyperthyroidism did not influence hippocampal neurogenesis, suggesting that the effects of thyroid hormone may be optimally permissive at euthyroid levels. Our in vivo and in vitro results revealed that adult hippocampal progenitors express thyroid receptor isoforms. The in vitro studies demonstrate that adult hippocampal progenitors exhibit enhanced proliferation, survival and glial differentiation in response to thyroid hormone. These results support a role for thyroid hormone in the regulation of adult hippocampal neurogenesis and raise the possibility that altered neurogenesis may contribute to the cognitive and behavioral deficits associated with adult-onset hypothyroidism. (c) 2005 Elsevier Inc. All rights reserved.