In situ fluorescence visualization of transcription sites and genomic Loci in blood stages of Plasmodium falciparum.
In situ fluorescence visualization of transcription sites and genomic Loci in blood stages of Plasmodium falciparum.
复制标题
DOI：
10.1007/978-1-62703-026-7_24
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Scherf A
中科院分区：
文献类型：
--
作者：
Mancio-Silva L;Scherf A
Fluorescence-based techniques have been used extensively in the malaria field to study the functional role of nuclear organization and gene positioning in blood stages of the human malaria parasite, Plasmodium falciparum. In this chapter, we present optimized protocols for bromouridine (BrUTP) incorporation into nascent RNA in live parasites and fluorescent in situ hybridization (FISH) in fixed parasites. Methodology to perform various combinations of the FISH assay, as well as a basic approach for quantitative analysis of nuclear position, is also described.
