Automated cerebrospinal fluid cell count - New reference ranges and evaluation of its clinical use in central nervous system infections
Automated cerebrospinal fluid cell count - New reference ranges and evaluation of its clinical use in central nervous system infections
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DOI：
10.1016/j.clinbiochem.2013.11.010
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发表时间：
2014-01-01
影响因子：
2.8
通讯作者：
Hagberg, Lars
中科院分区：
文献类型：
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作者：
Bremell, Daniel;Mattsson, Niklas;Hagberg, Lars
目的：本研究的目的是建立新的参考范围的白细胞在CSF和检查，如果单核细胞分离成淋巴细胞和单核细胞可以用来区分各种中枢神经系统感染，目前与一个类似的图片在手动CSF细胞counts.Design和方法：自动化细胞计数器西门子ADVIA 2120 i。对于参考范围部分，我们分析了80名神经系统健康志愿者的CSF。对于鉴别诊断部分，我们分析了细胞计数和175例CSF单核细胞pleocytosis.Results患者的医院记录：红细胞计数< 250个细胞/μ L的样本，自动和手动白细胞计数之间的相关性良好。对于参考范围章节中研究的神经系统健康志愿者，第95百分位数为淋巴细胞3.0个细胞/μ L，单核细胞1.0个细胞/μ L，粒细胞1.0个细胞/μ L。在鉴别诊断部分，对莱姆病神经疏螺旋体病和病毒性CNS感染进行了比较。这两组之间在细胞计数方面没有显著差异;淋巴细胞的中位数也是58个细胞/μ L对72个细胞/μ L（P = n.s.）;单核细胞的中位数也为13个细胞/μ L vs. 16个细胞/μ L（P = n.s.）;对于粒细胞，中位数1个细胞/μ L vs. 2个细胞/μ L（P = n.s.）结论：我们建议新的CSF细胞计数参考范围为淋巴细胞< 4个细胞/μ L，单核细胞< 3个细胞/μ L，粒细胞< 3个细胞/μ L。单核细胞分离成淋巴细胞和单核细胞并没有促进莱姆病神经疏螺旋体病和病毒CNS感染之间的歧视。(C)2013年加拿大临床化学家协会。爱思唯尔公司出版All rights reserved.
Objectives: The purposes of this study were to establish new reference ranges for leukocytes in the CSF and to examine if the separation of mononuclear cells into lymphocytes and monocytes could be used to differentiate between various CNS infections that present with a similar picture in manual CSF cell counts.Design and methods: The automated cell counter Siemens ADVIA 2120i was used. For the reference range section, we analyzed CSF from 80 neurologically healthy volunteers. For the differential diagnosis section we analyzed cell counts and hospital records from 175 patients with CSF mononuclear pleocytosis.Results: Correlation was good between automated and manual leukocyte counts for samples with erythrocyte counts < 250 cells/mu L. For the neurologically healthy volunteers studied in the reference range section, the 95th percentile was 3.0 cells/mu L for lymphocytes, 1.0 cell/mu L for monocytes and 1.0 cell/mu L for granulocytes. In the differential diagnosis section, comparisons were done between the groups Lyme neuroborreliosis and viral CNS infection. There were no significant differences between these two groups regarding cell counts; neither for lymphocytes, median 58 cells/mu L vs. 72 cells/mu L (P = n.s.); nor for monocytes, median 13 cells/mu L vs. 16 cells/mu L (P = n.s.); nor for granulocytes, median 1 cell/mu L vs. 2 cells/mu L (P = n.s.)Conclusions: We suggest new CSF cell count reference ranges of < 4 cells/mu L for lymphocytes, < 3 cells/mu L for monocytes and < 3 cells/mu L for granulocytes. The separation of mononuclear cells into lymphocytes and monocytes did not facilitate the discrimination between Lyme neuroborreliosis and viral CNS infection. (C) 2013 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.