Bioactivity and antibacterial activity of strontium and silver ion releasing titanium
Bioactivity and antibacterial activity of strontium and silver ion releasing titanium
复制标题
锶银离子释放钛的生物活性和抗菌活性
DOI：
10.1002/jbm.b.34695
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
Matsuda Shuichi
中科院分区：
文献类型：
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作者：
Masamoto Kazutaka;Fujibayashi Shunsuke;Yamaguchi Seiji;Otsuki Bungo;Okuzu Yaichiro;Kawata Tomotoshi;Goto Koji;Shimizu Takayoshi;Shimizu Yu;Kawai Toshiyuki;Hayashi Makoto;Morizane Kazuaki;Imamura Masashi;Ikeda Norimasa;Takaoka Yusuke;Matsuda Shuichi
为了克服与骨科植入物松动和手术部位感染相关的问题，我们开发了一种新型钛（Ti）基材料，该材料可在碱和热处理的基础上释放锶和银离子（CaSrAg-Ti）。比较了商业纯Ti（cp-Ti）、仅释放Sr离子的Ti（CaSr-Ti）和新型CaSrAg-Ti材料的结果。进行力学测试以评价CaSrAg-Ti的体内结合性能，并在植入大鼠股骨后4周和8周测定组织学样本中的骨-植入物接触（BIC）比。此外，在24 h孵育期后，通过测定菌落形成单位，评价了该材料对甲氧西林耐药金黄色葡萄球菌（MSSA）的体外抗菌活性。此外，在大鼠皮下袋模型中植入后7天，在体内评价抗菌活性。在组织学标本中，骨与CaSrAg-Ti之间存在直接接触，并且在任何大鼠中均无明显的银沉着症体征。与cp‐Ti相比，CaSrAg-Ti的骨结合强度和BIC比在4周时分别增加了2.7倍和2.3倍，在8周时分别增加了2.2倍和2.0倍。与cp-Ti相比，CaSrAg-Ti上剩余的活MSSA数量在体外减少了100 ± 0%，在体内减少了94.2 ± 6.9%。释放Sr和Ag离子的Ti是一种具有骨结合性能和抗MSSA活性的有前途的材料。
To overcome problems associated with loosening of orthopedic implants and surgical site infections, we developed a novel, titanium (Ti)‐based material that releases both strontium and silver ions (CaSrAg–Ti) based on alkali‐and‐heat treatment. The results of commercially pure Ti (cp–Ti), Ti that releases Sr ions only (CaSr–Ti), and the novel CaSrAg–Ti material were compared. Mechanical tests were performed to evaluate the in vivo bonding properties of CaSrAg–Ti and the bone‐implant contact (BIC) ratio in histological specimens was determined at 4 and 8 weeks after implantation in a rat femur. Also, the in vitro antibacterial activities of this material against methicillin‐susceptibleStaphylococcus aureus(MSSA) were evaluated after a 24 h incubation period by assaying colony‐forming units. In addition, antibacterial activities were evaluated in vivo at 7 days after implantation in a rat subcutaneous pocket model. There was direct contact between the bone and CaSrAg–Ti in histological specimens and no apparent signs of argyrosis in any rat. The bone‐bonding strength and the BIC ratio were increased by 2.7‐ and 2.3‐fold for CaSrAg–Ti vs. cp‐Ti at 4 weeks and 2.2‐ and 2.0‐fold at 8 weeks, respectively. As compared with cp‐Ti, the number of viable MSSA remaining on CaSrAg–Ti was reduced by 100 ± 0% in vitro and 94.2 ± 6.9% in vivo. Ti that releases Sr and Ag ions is a promising material that exhibits both bone‐bonding properties and anti‐MSSA activities.