3-DIMENSIONAL MEMBRANE CRYSTALS IN AMPHIBIAN CONE OUTER SEGMENTS .2. CRYSTAL TYPE ASSOCIATED WITH THE SADDLE-POINT REGIONS OF CONE DISKS
3-DIMENSIONAL MEMBRANE CRYSTALS IN AMPHIBIAN CONE OUTER SEGMENTS .2. CRYSTAL TYPE ASSOCIATED WITH THE SADDLE-POINT REGIONS OF CONE DISKS
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DOI：
10.1016/s0014-4835(05)80128-9
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发表时间：
1995-09-01
影响因子：
3.4
通讯作者：
SCHNEIDER, TG
中科院分区：
文献类型：
--
作者：
CORLESS, JM;WORNIALLO, E;SCHNEIDER, TG
在刚果鳗蝾螈（Amphiuma）的光适应灌注固定视网膜中，我们观察到锥形外节盘轴向阵列内独特的三维晶体区域。这些晶体域，每个包含2-12个圆盘，已经在锥体外段的远端观察到，并且与鞍点区域和锥体周长的邻近部分相关联。在纵向切片中，晶体通常显示轴向取向的细胞质丝阵列，横向间距在12-13 nm范围内。椎间盘内腔室的宽度在晶体内扩展到8- 10nm，近似于细胞质腔室的宽度。在某些切片中，细胞质丝与盘内丝轴向排列，长度和宽度相似。在横截面上，投影的晶格看起来近似为矩形，单位胞尺寸类似于12 nm x 12.5 nm。在形状、方向、尺寸、位置、投影对称性和相关的膜间距关系上，锥形外段晶丝与沿杆盘周缘和边缘的晶格丝有很强的相似性。这些相似性表明，锥晶细丝可能与棒状圆盘的边缘蛋白有关。最后，这些晶体与鞍点区域的优先关联间接支持了这样的假设，即COS盘面积的减少与根尖位移是通过盘膜成分通过鞍点的吸收来完成的。1995学术出版社有限公司
In light-adapted, perfusion fixed retinas of the Congo eel salamander, Amphiuma, we have observed distinctive 3-D crystalline domains within the axial array of cone outer segment disks. These crystalline domains, each involving 2-12 disks, have been observed in the distal half of cone outer segment, and are associated with saddle point regions and immediately adjacent segments of the cone disk perimeter, In longitudinal sections, the crystals typically display an axially oriented array of cytoplasmic filaments with lateral spacings in the range of 12-13 nm. The width of the intradiskal compartment is expanded to 8-10 nm within the crystal, and approximates the width of the cytoplasmic compartment. In some sections, the cytoplasmic filaments are axially aligned with intradiskal filaments of similar length and width. In transverse sections, the projected lattice appears to be approximately rectangular, with unit cell dimensions of similar to 12 nm x 12.5 nm. In shape, orientation, dimension, location, projection symmetry and associated membrane spacing relationships, the cone outer segment crystal filaments share a strong resemblance with lattice filaments located along the perimeters and preincisures of rod disks. These similarities suggest that the cone crystal filaments may be related to the rim protein of rod disks. Lastly, the preferential association of these crystals with saddle point regions indirectly supports the hypothesis that reductions in COS disk area with apical displacement are accomplished by resorption of disk membrane components through the saddle points. (C) 1995 Academic Press Limited.