Antimicrobial Responses in the Male Reproductive Tract of Lipopolysaccharide Challenged Rats
Antimicrobial Responses in the Male Reproductive Tract of Lipopolysaccharide Challenged Rats
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DOI：
10.1111/j.1600-0897.2010.00937.x
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发表时间：
2011-06-01
影响因子：
3.6
通讯作者：
Yenugu, Suresh
中科院分区：
文献类型：
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作者：
Biswas, Barnali;Yenugu, Suresh
问题包括Toll样受体（TLR）在内的先天免疫机制赋予了对抗进入体内的病原微生物的第一道防线机制。男性生殖道是容易受到感染和TLR的作用和抗微生物的反应，在这个器官systems.Method的反感染的操作是知之甚少StudyCaput和尾部附睾，睾丸和精囊收集在0，3，6，9，12，15和24小时从大鼠腹腔注射单剂量的LPS。采用ELISA法测定血浆睾酮。采用RT-PCR分析防御素和Spag 11亚型的表达模式。进行免疫组织化学分析，以确定SPAG 11 E蛋白表达后LPStreatment.ResultsWe提供的第一条证据表明，男性生殖道诱导表达的精子相关抗原11（Spag 11）的mRNA变体和防御素时，挑战与脂多糖（LPS）伴随蛋白表达的增加。然而，抗菌基因表达的诱导和血浆睾酮之间呈负相关。观察到的促炎细胞因子的mRNA水平的增加平行的Spag 11变体和大多数的防御素的表达在男性reproductive tract.ConclusionThe响应于LPS管理的Spag 11和防御素mRNA的增加表明其重要性，在感染期间保护男性生殖道。本研究的结果有助于了解男性生殖道先天免疫防御机制，并设计新的肽类抗生素来预防性传播疾病。
ProblemInnate immune machinery including the Toll-like receptors (TLRs) confers the first line of defense mechanisms to counter pathogenic microorganisms that enter the body. The male reproductive tract is vulnerable to infection and the role of TLRs and the antimicrobial responses that operate to counter infections in this organ system are poorly understood.Method of StudyCaput and cauda epididymides, testes and seminal vesicles were collected at 0, 3, 6, 9, 12, 15 and 24 h from rats injected intraperitoneally with a single dose of LPS. Plasma testosterone was measured using ELISA. Expression pattern of defensins and Spag11 isoforms were analysed using RT-PCR. Immunohistochemical analyses was performed to determine SPAG11E protein expression following LPS treatment.ResultsWe provide the first line of evidence that the male reproductive tract induces the expression of Sperm Associated Antigen 11 (Spag11) mRNA variants and defensins when challenged with lipopolysaccharide (LPS) with a concomitant increase in protein expression. However, there was an inverse relationship between induction of antimicrobial gene expression and plasma testosterone. An increase in the mRNA levels of proinflammatory cytokines was observed parallel to the induction of Spag11 variants and majority of defensin expression in the male reproductive tract.ConclusionThe increase in Spag11 and defensin mRNA in response to LPS administration demonstrates their importance in protecting the male reproductive tract during infection. Results of this study help to understand male reproductive tract innate immune defense mechanisms and to design novel peptide antibiotics to prevent sexually transmitted diseases.