Electrodeless dielectrophoresis of single- and double-stranded DNA
Electrodeless dielectrophoresis of single- and double-stranded DNA
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DOI：
10.1016/s0006-3495(02)73977-5
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发表时间：
2002-10-01
影响因子：
3.4
通讯作者：
Austin, RH
中科院分区：
文献类型：
--
作者：
Chou, CF;Tegenfeldt, JO;Austin, RH
分子的介电泳捕获通常使用金属电极来进行以提供高场梯度。在本文中，我们证明了介电泳捕获使用绝缘收缩在远低于频率比金属捕获结构，因为水电解是可行的。我们证明了无电极介电电泳（EDEP）可以用于单链和双链DNA的浓缩和图案化。基于所观察到的捕获力的频率、粘度和场依赖性，讨论了离子溶液中DNA极化的可能机制。
Dielectrophoretic trapping of molecules is typically carried out using metal electrodes to provide high field gradients. In this paper we demonstrate dielectrophoretic trapping using insulating constrictions at far lower frequencies than are feasible with metallic trapping structures because of water electrolysis. We demonstrate that electrodeless dielectrophoresis (EDEP) can be used for concentration and patterning of both single-strand and double-strand DNA. A possible mechanism for DNA polarization in ionic solution is discussed based on the frequency, viscosity, and field dependence of the observed trapping force.