Fission yeast Pom1p kinase activity is cell cycle regulated and essential for cellular symmetry during growth and division
Fission yeast Pom1p kinase activity is cell cycle regulated and essential for cellular symmetry during growth and division
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DOI：
10.1093/emboj/20.5.1064
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发表时间：
2001-03-01
期刊：
影响因子：
11.4
通讯作者：
Nurse, P
中科院分区：
文献类型：
--
作者：
Bähler, J;Nurse, P
裂殖酵母细胞在分裂间期的大部分时间内从两端生长，对称分裂为两个子细胞。pom1基因，编码蛋白激酶Dyrk家族的成员，已被确定通过突变体显示异常的细胞形态发生。在这里，我们表明，Pom1p激酶活性是细胞周期调节的相关性，与细胞对称性的状态：活动是高的对称生长和分裂，但较低时，细胞生长在一端。催化结构域中的点突变导致细胞生长和分裂过程中的不对称性，而过表达Pom1p的细胞形成额外的生长末端。激酶活性的操作表明Pom1p在细胞末端微管生长中的负面作用。Pom1p存在于一个大的蛋白质复合物中，需要其非催化结构域定位于细胞外周，并需要其激酶活性定位于细胞末端。这些数据表明，Pom1p激酶活性在产生细胞对称性中起着重要作用，并表明在所有真核生物中普遍存在的同源蛋白激酶可能具有相关作用。
Schizosaccharomyces pombe cells grow from both ends during most of interphase and divide symmetrically into two daughter cells. The pom1 gene, encoding a member of the Dyrk family of protein kinases, has been identified through a mutant showing abnormal cellular morphogenesis. Here we show that Pom1p kinase activity is cell cycle regulated in correlation,vith the state of cellular symmetry: the activity is high during symmetrical growth and division, but lower when cells grow at just one end. Point mutations in the catalytic domain lead to asymmetry during both cell growth and division, whilst cells overexpressing Pom1p form additional growing ends. Manipulations of kinase activity indicate a negative role for Pom1p in microtubule growth at cell ends. Pom1p is present in a large protein complex and requires its non-catalytic domain to localize to the cell periphery and its kinase activity to localize to cell ends. These data establish that Pom1p kinase activity plays an important role in generating cellular symmetry and suggest that there may be related roles of homologous protein kinases ubiquitously present in all eukaryotes.