Fourier transform infrared/vibrational circular dichroism spectroscopy as an informative tool for the investigation of large supramolecular complexes of biological macromolecules
Fourier transform infrared/vibrational circular dichroism spectroscopy as an informative tool for the investigation of large supramolecular complexes of biological macromolecules
复制标题
傅里叶变换红外/振动圆二色光谱作为研究生物大分子超分子复合物的信息工具
DOI：
10.1002/bip.20299
复制
发表时间：
2005
期刊：
影响因子：
2.9
通讯作者：
H. Wieser
中科院分区：
文献类型：
--
作者：
A. Polyanichko;H. Wieser
利用紫外、红外吸收光谱和圆二色光谱研究了大分子DNA-蛋白质复合物的结构和形成过程。这种技术组合用于克服由于此类溶液中相当大的光散射而导致的UV-CD（电子或ECD）光谱的局限性。基于FTIR和UV‐CD光谱分析，研究了DNA与非组蛋白染色质蛋白HMGB 1和连接蛋白H1的相互作用。获得的数据显示，在实验条件下（15 mM NaCl，蛋白质/DNA比率r < 1 w/w），蛋白质在结合DNA时没有显示任何AT或GC特异性。在两种蛋白质的存在下，主要在DNA小沟中观察到相互作用，这归因于HMGB 1结合。组蛋白H1通过与糖-磷酸骨架的带负电荷基团相互作用以及HMGB 1的天冬氨酸和谷氨酸氨基酸残基的结合促进HMGB 1与DNA的结合。HMGB 1和H1共同作用，刺激超分子DNA-蛋白质结构的组装。三元复合物的结构组织不仅取决于蛋白质-DNA相互作用的性质，而且还取决于HMGB 1和H1分子之间的相互作用。© 2005 Wiley Periodicals，Inc. Biopolymers 78：329-339，2005
A combination of ultraviolet (UV) and infrared (IR) absorption and circular dichroism (CD) spectroscopy was applied to investigate the structure and formation of large supramolecular DNA–protein complexes. This combination of techniques was used to overcome limitations of UV‐CD (electronic, or ECD) spectroscopy due to considerable light scattering in such solutions. Based on the analysis of FTIR and UV‐CD spectra, the interaction of DNA with nonhistone chromatin protein HMGB1 and linker histone H1 was studied. The data obtained showed that under the conditions of the experiment (15 mM NaCl, protein/DNA ratio r < 1 w/w) the proteins did not reveal any AT or GC specificity in binding to DNA. In the presence of both proteins, mainly interactions in the DNA minor groove were observed, which were attributed to HMGB1 binding. Histone H1 facilitated binding of HMGB1 to DNA by interacting with the negatively charged groups of the sugar–phosphate backbone and binding of aspartic and glutamic amino acid residues of HMGB1. Acting together, HMGB1 and H1 stimulated the assemblage of supramolecular DNA–protein structures. The structural organization of the ternary complexes depended not only on the properties of the protein–DNA interactions but also on the interactions between HMGB1 and H1 molecules. © 2005 Wiley Periodicals, Inc. Biopolymers 78: 329–339, 2005
影响因子：
2.9
作者：
Love, JJ;Li, X;Wright, PE
通讯作者：
Wright, PE
偏振光生物分子结构的吸收、散射和成像。
DOI：
10.1146/annurev.bb.16.060187.001535
发表时间：
1987
期刊：
Annual review of biophysics and biophysical chemistry
影响因子：
--
作者：
TinocoJr,I;Mickols,W;Maestre,MF;Bustamante,C
通讯作者：
Bustamante,C
非组蛋白染色体蛋白 HMG1 调节组蛋白 H1 诱导的 DNA 浓缩。
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kohlstaedt,LA;Sung,EC;Fujishige,A;Cole,RD
通讯作者：
Cole,RD