Red clover (Trifolium pratense L.) draft genome provides a platform for trait improvement.
Red clover (Trifolium pratense L.) draft genome provides a platform for trait improvement.
复制标题
DOI：
10.1038/srep17394
复制
发表时间：
2015-11-30
影响因子：
4.6
通讯作者：
Skøt L
中科院分区：
文献类型：
--
作者：
De Vega JJ;Ayling S;Hegarty M;Kudrna D;Goicoechea JL;Ergon Å;Rognli OA;Jones C;Swain M;Geurts R;Lang C;Mayer KF;Rössner S;Yates S;Webb KJ;Donnison IS;Oldroyd GE;Wing RA;Caccamo M;Powell W;Abberton MT;Skøt L
三叶草（Trifolium pratense L.）是全球重要的牧草豆科牧草在畜牧养殖系统。它是草地农业的一个有吸引力的组成部分，因为它的产量和蛋白质含量高，营养价值和固定大气氮的能力。进一步加强其在可持续农业中的作用需要遗传改良持久性，抗病性和耐放牧性。为了帮助解决这些挑战，我们已经为红三叶草组装了一个染色体规模的参考基因组。我们观察到大块的保守同线性与蒺藜苜蓿，并估计这两个物种分歧约2300万年前。在40，868个注释基因中，我们确定了参与牧草质量和牲畜营养重要生化途径的基因簇。通过对86种基因型的合成群体进行测序的基因型分析表明，基于基因组学的育种方法所需的标记数量是易处理的，这使得红三叶草成为关联研究和基因组选择的合适候选者。
Red clover (Trifolium pratense L.) is a globally significant forage legume in pastoral livestock farming systems. It is an attractive component of grassland farming, because of its high yield and protein content, nutritional value and ability to fix atmospheric nitrogen. Enhancing its role further in sustainable agriculture requires genetic improvement of persistency, disease resistance, and tolerance to grazing. To help address these challenges, we have assembled a chromosome-scale reference genome for red clover. We observed large blocks of conserved synteny with Medicago truncatula and estimated that the two species diverged ~23 million years ago. Among the 40,868 annotated genes, we identified gene clusters involved in biochemical pathways of importance for forage quality and livestock nutrition. Genotyping by sequencing of a synthetic population of 86 genotypes show that the number of markers required for genomics-based breeding approaches is tractable, making red clover a suitable candidate for association studies and genomic selection.