Nontuberculous mycobacteria in gastrostomy fed patients with cystic fibrosis.
Nontuberculous mycobacteria in gastrostomy fed patients with cystic fibrosis.
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DOI：
10.1038/srep46546
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发表时间：
2017-04-24
影响因子：
4.6
通讯作者：
Ward C
中科院分区：
文献类型：
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作者：
Al-Momani H;Perry A;Jones R;Bourke S;Doe S;Perry J;Anderson A;Forrest T;Forrest I;Griffin M;Brodlie M;Pearson J;Ward C
多药耐药结核分枝杆菌复合体（MABSC）是一种非结核分枝杆菌（NTM），在囊性纤维化（CF）中受到国际关注。我们假设胃液和经皮内镜胃造口术（PEG）喂食装置可能产生MABSC分离株。研究了16例成人PEG喂养CF患者和5例更换PEG管的胃液和尿液。培养细菌和真菌分离株。通过rpoB、sodA和hsp 65基因测序鉴定分枝杆菌，并使用可变数目串联重复序列进行菌株分型。所有胃液、痰液和PEG样本中都生长出细菌和/或真菌。7例患者检测到MABSC。5例痰中有MABSC。2例患者的痰液和胃液中分离出相同的MABSC菌株，1例患者的PEG管和痰液中分离出相同的菌株。2例痰液样本阴性的患者在其胃液或PEG管中分离出MABSC。因此，首次从少数患者的胃样本中鉴定出MABSC。我们的结论是，胃液和聚乙二醇管可能是CF患者中MABSC分离株的潜在来源，这些发现值得进一步研究。
Multi-drug resistant Mycobacterium abscessus complex (MABSC) is a form of Nontuberculous mycobacteria (NTM) of special, international concern in Cystic Fibrosis (CF). We hypothesised that gastric juice and percutaneous endoscopic gastrostomy (PEG) feeding devices might yield MABSC isolates. Gastric juice and sputa from sixteen adult PEG fed CF patients and five replaced PEG tubes were studied. Bacterial and fungal isolates were cultured. Mycobacterium were identified by rpoB, sodA and hsp65 gene sequencing and strain typed using variable number tandem repeat. Bacteria and/or fungi grew from all gastric juice, sputa and PEG samples. MABSC were detected in 7 patients. Five had MABSC in their sputum. Two had an identical MABSC strain in their sputum and gastric juice and one had the same strain isolated from their PEG tube and sputum. Two patients who were sputum sample negative had MABSC isolated in their gastric juice or PEG tube. MABSC were therefore identified for the first time from a gastric sample in a minority of patients. We conclude that gastric juice and PEG-tubes may be a potential source of MABSC isolates in CF patients, and these findings warrant further study.