Brain cholecystokinin and nutritional status in rats and mice.
Brain cholecystokinin and nutritional status in rats and mice.
复制标题
大鼠和小鼠的脑胆囊收缩素和营养状况。
DOI：
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发表时间：
1979
影响因子：
15.9
通讯作者：
J. Hirsch
中科院分区：
文献类型：
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作者：
B. S. Schneider;J. Monahan;J. Hirsch
在一定条件下，外源性给予CCK或其COOH末端八肽可终止动物的摄食并引起行为饱足感。此外，通常在脊椎动物的大脑中发现高浓度的CCK。因此，人们假设大脑CCK在控制食欲方面起着作用。为了探索这种可能性，采用COOH末端放射免疫法测定了不同营养操作后大鼠和小鼠大脑皮层、下丘脑和脑干中CCK的浓度。CCK主要以其COOH末端八肽的形式存在于出生前不久的大鼠大脑中，并在出生前5周内在大脑皮层和脑干中迅速增加。早期严重营养不良对大鼠脑内CCK正常发育模式无影响。用Sephadex凝胶渗滤法测定，禁食72h的成年大鼠和高摄食大鼠的脑CCK浓度和CCK分子形式的分布没有改变。在四种遗传性肥胖啮齿类动物的大脑中也发现了正常的CCK浓度，在六种通过手术或化学损毁下丘脑腹内侧而变得过度肥胖的动物的大脑中也发现了正常的CCK浓度。结论是，尽管大鼠和小鼠的营养状况有极大的差异，但大脑主要结构中的CCK浓度保持着显著的稳定性。
Under certain conditions, exogenously administered cholecystokinin (CCK) or its COOH-terminal octapeptide can terminate feeding and cause behavioral satiety in animals. Furthermore, high concentrations of CCK are normally found in the brains of vertebrate species. It has thus been hypothesized that brain CCK plays a role in the control of appetite. To explore this possibility, a COOH-terminal radioimmunoassay was used to measure concentrations of CCK in the cerebral cortex, hypothalamus, and brain stem of rats and mice after a variety of nutritional manipulations. CCK, mainly in the form of its COOH-terminal octapeptide, was found to appear in rat brain shortly before birth and to increase rapidly in cortex and brain stem throughout the first 5 wk of life. Severe early undernutrition had no effect on the normal pattern of CCK development in rat brain. Adult rats deprived of food for up to 72 h and rats made hyperphagic with highly palatable diets showed no alterations in brain CCK concentrations or distribution of molecular forms of CCK as determined by Sephadex gel filtration of brain extracts. Normal CCK concentrations were also found in the brains of four strains of genetically obese rodents and in the brains of six animals made hyperphagic and obese by surgical or chemical lesioning of the ventromedial hypothalamus. It is concluded that despite extreme variations in the nutritional status of rats and mice, CCK concentrations in major structures of the brain are maintained with remarkable constancy.