Electron microscopic demonstration of tyrosine hydroxylase-immunoreactive interplexiform cells in the lamprey retina
Electron microscopic demonstration of tyrosine hydroxylase-immunoreactive interplexiform cells in the lamprey retina
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DOI：
10.1016/0304-3940(96)12511-8
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发表时间：
1996-04-05
影响因子：
2.5
通讯作者：
NguyenLegros, J
中科院分区：
文献类型：
--
作者：
DalilThiney, N;VersauxBotteri, C;NguyenLegros, J
为了澄清关于七鳃鳗视网膜中是否存在多巴胺能丛状细胞的争议，我们使用抗酪氨酸羟化酶抗体对河七鳃鳗的视网膜进行了免疫细胞化学电子显微镜研究。我们证明了内层（IPL）和外层（OPL）丛状层中都存在免疫反应过程。外部突起与 OPL 中的水平细胞突起和光感受器末梢紧密接触，但不形成经典突触。其中一些被包裹在米勒细胞的细胞质内。因此，多巴胺能丛状细胞确实存在于河灯中，但突触的缺乏反而支持多巴胺在外视网膜中的旁分泌作用。
To clarify the controversies about the existence (or not) of dopaminergic interplexiform cells in the lamprey retina, we have performed an immunocytochemical electron microscopic study of the retina of the river lamprey Lampetra fluviatilis, using anti-tyrosine hydroxylase antibody. We demonstrate the presence of immunoreactive processes in both inner (IPL) and outer (OPL) plexiform layers. The external processes are in close contact with horizontal cell processes and photoreceptor terminals in the OPL, but do not make classical synapses. Some of them are ensheathed within Muller cell cytoplasm. Thus, dopaminergic interplexiform cells do exist in Lampetra fluviatilis, but the absence of synapses rather supports a paracrine action of dopamine in the outer retina.