The minimum surface fibrinogen concentration necessary for platelet activation on dimethyldichlorosilane-coated glass.
The minimum surface fibrinogen concentration necessary for platelet activation on dimethyldichlorosilane-coated glass.
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DOI：
10.1002/jbm.820250311
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发表时间：
1991-03
期刊：
影响因子：
--
通讯作者：
K. Park;F. Mao;H. Park
中科院分区：
文献类型：
--
作者：
K. Park;F. Mao;H. Park
白蛋白和纤维蛋白原竞争吸附到二甲基二氯硅烷涂层的玻璃（DDS-玻璃）和血小板活化作为表面纤维蛋白原浓度的函数进行了检查。吸附溶液中白蛋白与纤维蛋白原的重量比在10至700之间变化。血小板活化通过铺展的血小板的面积和圆度来定量。当DDS-玻璃仅用白蛋白包被时，血小板仅接触粘附，根本不能扩散。然而，在纤维蛋白原和白蛋白的竞争吸附之后，血小板能够在表面上扩散。当表面纤维蛋白原浓度增加至0.02微克/厘米2时，血小板活化呈线性增加。如果表面纤维蛋白原浓度为0.02 μ g/cm 2或更高，即使表面由白蛋白主导，血小板也能够完全活化。似乎只要血小板表面的一小部分（2-15%）被紧密结合的纤维蛋白原覆盖，血小板就可以完全活化。
Albumin and fibrinogen were competitively adsorbed onto dimethyldichlorosilane-coated glass (DDS-glass) and platelet activation was examined as a function of the surface fibrinogen concentration. The weight ratio of albumin to fibrinogen in the adsorption solution was varied from 10 to 700. Platelet activation was quantitated by the area and circularity of spread platelets. When the DDS-glass was coated with albumin alone, platelets were only contact adherent and could not spread at all. After competitive adsorption of fibrinogen and albumin, however, platelets were able to spread on the surface. Platelet activation increased linearly as the surface fibrinogen concentration increased up to 0.02 micrograms/cm2. Platelets were able to activate fully if the surface fibrinogen concentration was 0.02 micrograms/cm2 or higher, even though the surface was dominated by albumin. It appears that platelets can activate fully as long as only a small fraction (2-15%) of the surface is covered with tightly bound fibrinogen.