ULTRASTRUCTURE OF MOUSE VALLATE TASTE-BUDS .2. CELL-TYPES AND CELL LINEAGE
ULTRASTRUCTURE OF MOUSE VALLATE TASTE-BUDS .2. CELL-TYPES AND CELL LINEAGE
复制标题
DOI：
10.1002/cne.902530210
复制
发表时间：
1986-11-08
影响因子：
2.5
通讯作者：
ROPER, SD
中科院分区：
文献类型：
--
作者：
DELAY, RJ;KINNAMON, JC;ROPER, SD
用高压电子显微镜(HVEM)放射自显影技术(ARG)观察单次注射~(3+)-胸腺嘧啶核苷对小鼠味蕾细胞寿命的影响。分别于注射后1小时、6小时、12小时、24小时处死动物，连续10天处死动物。制备舌组织作HVEM-ARG，以便对标记细胞进行鉴定和表征。味觉细胞分为四类：基细胞、暗细胞、中间细胞和亮细胞。基底细胞是位于味蕾基侧附近的多边形细胞，其主要特征是核膜上有细丝附着。暗细胞和亮细胞具有前人所描述的典型特征。中间细胞的特征介于暗细胞和亮细胞之间。在注射3 H-胸腺嘧啶核苷后的前2天，90%以上的标记细胞为基底细胞。标记暗细胞在注射后6h开始出现，在注射后第4d达到高峰，然后逐渐下降。标记的中间细胞出现在暗细胞出现后(12h)，在注射~3H-TdR后第5天达到高峰。最后，在注射后第4天开始观察到标记的轻细胞，并持续增加，直到第10天，它们占标记细胞的45%。这些数据支持这样一种假设，即在小鼠的凹凸状味蕾中，有一种细胞系在其寿命期间经历了形态变化。
The lifespan of cells in the mouse taste bud was examined with high-voltage electron microscopic (HVEM) autoradiography (ARG) after giving a single injection of 3H-thymidine. Animals were killed at 1 hour, 6 hours, 12 hours, 24 hours, and then daily up through 10 days postinjection. Lingual tissues were prepared for HVEM ARG so that we could identify and characterized labeled cells. Four categories of taste cells were identified: basal, dark, intermediate, and light cells. Basal cells were polygonal cells located near the basolateral sides of the taste buds and were characterized primaro;y by the presence of filaments attached to the nuclear envelope. Dark and light cells had the typical features described by previous authors. Intermediate cells had features in between those of dark and light cells. Over 90% of the cells labeled in the first 2 days following injection of 3H-thymidine were basal cells. Labeled dark cells appeared 6 hours after injection, reached their peak incidence at the fourth day postinjection, and then gradually decreased. Labeled intermediate cells were identified after the appearance of dark cells (12 hour) and reached a peak incidence at the fifth day after injection of 3H-thymidine. Lastly, labeled light cells were first observed on the fourth day postinjection and continued to increase until the tenth day, when they constituted 45% of the labeled cells. These data support the hypothesis that there is one cell line in the mouse vallate taste bud that undergoes morphological changes in its lifespan.