ENO2Affects the Seed Size and Weight by Adjusting Cytokinin Content and Forming ENO2-bZIP75 Complex inArabidopsis thaliana
ENO2Affects the Seed Size and Weight by Adjusting Cytokinin Content and Forming ENO2-bZIP75 Complex inArabidopsis thaliana
复制标题
ENO2通过调节拟南芥细胞分裂素含量和形成ENO2-bZIP75复合物影响种子大小和重量
DOI：
10.3389/fpls.2020.574316
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发表时间：
2020-08-26
影响因子：
5.6
通讯作者：
Zhang, Genfa
中科院分区：
文献类型：
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作者：
Liu, Zijin;Zheng, Lamei;Zhang, Genfa
拟南芥ENO2(AtENO2)编码两种蛋白AtENO2(烯醇化酶)和AtMBP-1(c-Myc结合蛋白1)。AtENO2功能的丧失会导致结构性发育缺陷，这种缺陷与烯醇化酶活性降低有关，但与AtMBP-1转录本丰度无关。然而，AtEO2对种子特性的调节机制尚不清楚。在本研究中，我们发现AtEO2的突变使种子的大小和重量减小。与WT植株相比，AtENO2突变体的籽粒中葡萄糖含量显著升高，而淀粉含量显著降低。我们还发现，AtEO2突变降低了细胞分裂素的含量，从而导致子叶变小。RNA-seq数据显示，差异表达基因有1892个，次生代谢途径明显丰富。AtENO2蛋白不是与AtMBP-1相互作用，而是与AtbZIP75相互作用，可能参与次生代谢。因此，ENO2改变了种子的大小和重量，这不仅受细胞分裂素含量和次生代谢的调节，还可能受到ENO2和bZIP57相互作用的影响。这些结果有助于理解AtENO2的新功能，为进一步挖掘作物育种的关键候选基因奠定了基础。
Arabidopsis thaliana ENO2(AtENO2) encodes two proteins AtENO2 (enolase) and AtMBP-1 (c-Myc binding protein 1-like). The loss ofAtENO2function causes the constitutive developmental defects which are correlated with reduced enolase activity, but not AtMBP-1 transcript abundance. However, the regulation mechanism ofAtENO2on the seed properties is still not clear. In this study, we found that the mutation ofAtENO2reduced the seed size and weight. The level of glucose in seed was significantly elevated but that of starch was decreased inAtENO2mutants compared to WT plants. We also found thatAtENO2mutation reduced the content of cytokinin which resulted in smaller cotyledons. The RNA-seq data showed that there were 1892 differentially expressed genes and secondary metabolic pathways were significantly enriched. Instead of AtMBP-1, AtENO2 protein interacted with AtbZIP75 which may mediate the secondary metabolism. Therefore,ENO2alters the size and weight of seeds which is not only regulated by the content of cytokinin and secondary metabolism, but may be affected by the interaction of ENO2 and bZIP57. These results are helpful to understand the novel function ofAtENO2which provide a foundation for further exploration of the key candidate genes for crop breeding.