Synergistic effects of adjuvants interferon-gamma and levamisole on DNA vaccination against infection with Newcastle disease virus.
Synergistic effects of adjuvants interferon-gamma and levamisole on DNA vaccination against infection with Newcastle disease virus.
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DOI：
10.1089/vim.2006.0108
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发表时间：
2007-06
期刊：
影响因子：
2.2
通讯作者：
Jiangmei Yin;Huali Jin;Fu Yang;Zheng Ding;Chang-Li Huang;Qinghong Zhu;Bin Wang
中科院分区：
文献类型：
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作者：
Jiangmei Yin;Huali Jin;Fu Yang;Zheng Ding;Chang-Li Huang;Qinghong Zhu;Bin Wang
体液和细胞介导的免疫应答对于保护动物免受初始急性病毒感染和建立慢性感染都是重要的。DNA疫苗的佐剂可以影响体液免疫和细胞免疫之间的平衡。本研究以鸡干扰素（provax-chIFN-γ）cDNA为分子佐剂，左旋咪唑（LMS）为化学佐剂，构建了含纽卡斯尔病病毒（NDV）血凝素-神经氨酸酶基因和融合基因的DNA疫苗，检测其对NDV致死性攻击的免疫效果。与单独的DNA疫苗相比，具有provax-chIFN-γ加LMS的DNA疫苗诱导了显著更高的体液和细胞介导的应答，如更高水平的血凝抑制（HI）滴度和T细胞增殖所示。此外，具有provax-chIFN-γ加LMS制剂的DNA疫苗增加了IFN-γ、白细胞介素（IL）-2、IL-4、IL-12和IL-13的表达，表明IFN-γ和LMS制剂的有效性部分是由于平衡的细胞因子产生的增强。此外，这两种佐剂在鸡中产生了80%的保护作用，抵抗致死剂量的强毒NDV毒株的攻击。本研究表明，provax-chIFN-γ与LMS作为NDV DNA疫苗佐剂的协同效应可用于提高鸡的保护效果。
Both humoral and cell-mediated immune responses are important to protect animals from initial acute viral infection and establishment of chronic infection. Adjuvants for DNA vaccines can influence the balance between humoral and cell-mediated immunities. In this study, a DNA vaccine encoding the hemagglutinin-neuraminidase and fusion genes of Newcastle disease virus (NDV) incorporated with chicken interferon(provax-chIFN-gamma) cDNA as a molecular adjuvant and levamisole (LMS) as a chemical adjuvant was tested for its efficacy in protection against NDV lethal challenge. Compared with DNA vaccine alone, the DNA vaccine with provax-chIFN-gamma plus LMS induced significantly higher humoral and cell-mediated responses, as shown by higher levels of hemagglutination inhibition (HI) titers and T cell proliferation. In addition, the DNA vaccine with provax-chIFN-gamma plus LMS formulation increased the expression of IFN-gamma, interleukin (IL)-2, IL-4, IL-12, and IL-13, suggesting that the effectiveness of the IFN-gamma and LMS formulation is partly due to the enhancement of balanced cytokine production. Furthermore, the two adjuvants yielded 80% protection in chickens against challenge with a lethal dose of the virulent NDV strain. This study demonstrates that the synergistic effects of provax-chIFN-gamma plus LMS as the adjuvants in NDV DNA vaccination could be used to improve protective efficacy in chickens.