Antimicrobial drug-resistant Escherichia coli from humans and poultry products, Minnesota and Wisconsin, 2002-2004.
Antimicrobial drug-resistant Escherichia coli from humans and poultry products, Minnesota and Wisconsin, 2002-2004.
复制标题
DOI：
10.3201/eid1306.061576
复制
发表时间：
2007-06
影响因子：
11.8
通讯作者：
Belongia EA
中科院分区：
文献类型：
--
作者：
Johnson JR;Sannes MR;Croy C;Johnston B;Clabots C;Kuskowski MA;Bender J;Smith KE;Winokur PL;Belongia EA
耐药E.人类和家禽产品中的大肠杆菌。食物供应，包括家禽产品，可能会将耐药性大肠杆菌传播给人类。为了评估这一假设，931地理和时间匹配的E。应用PCR技术对从健康人（住院病人和健康素食者）和市售家禽产品（常规饲养和不使用抗菌药物饲养）中分离的大肠杆菌菌株进行系统发育群（A、B1、B2、D）和60个与肠外致病性大肠杆菌相关的毒力基因检测。杆菌对甲氧苄啶-磺胺甲恶唑、喹诺酮类和超广谱头孢菌素耐药的菌株（n = 331）与按来源分层的药物敏感菌株（n = 600）进行比较。药物敏感的人分离株的系统发育和毒力标记与人和家禽分离株的系统发育和毒力标记有很大不同。相比之下，耐药人分离株与家禽分离株相似，药物敏感和耐药家禽分离株在很大程度上无法区分。许多耐药的人类粪便E.大肠杆菌分离株可能来源于家禽，而耐药家禽源大肠杆菌。大肠杆菌分离株可能来源于易感禽类来源的前体。
Similarities were found between drug-resistant E. coli from humans and poultry products. The food supply, including poultry products, may transmit antimicrobial drug–resistant Escherichia coli to humans. To assess this hypothesis, 931 geographically and temporally matched E. coli isolates from human volunteers (hospital inpatients and healthy vegetarians) and commercial poultry products (conventionally raised or raised without antimicrobial drugs) were tested by PCR for phylogenetic group (A, B1, B2, D) and 60 virulence genes associated with extraintestinal pathogenic E. coli. Isolates resistant to trimethoprim-sulfamethoxazole, quinolones, and extended-spectrum cephalosporins (n = 331) were compared with drug-susceptible isolates (n = 600) stratified by source. Phylogenetic and virulence markers of drug-susceptible human isolates differed considerably from those of human and poultry isolates. In contrast, drug-resistant human isolates were similar to poultry isolates, and drug-susceptible and drug-resistant poultry isolates were largely indistinguishable. Many drug-resistant human fecal E. coli isolates may originate from poultry, whereas drug-resistant poultry-source E. coli isolates likely originate from susceptible poultry-source precursors.