Conditions for gene disruption by homologous recombination of exogenous DNA into the Sulfolobus solfataricus genome
Conditions for gene disruption by homologous recombination of exogenous DNA into the Sulfolobus solfataricus genome
复制标题
DOI：
10.1155/2008/948014
复制
发表时间：
2008-12-01
期刊：
影响因子：
2.4
通讯作者：
Driessen, Arnold J. M.
中科院分区：
文献类型：
--
作者：
Albers, Sonja-Verena;Driessen, Arnold J. M.
定向基因缺失突变体的构建是分子生物学中的一个重要工具，它允许功能研究油基因在其自然环境中的作用。对于极端嗜热古菌，一直难以获得可靠的系统来构建这样的突变体。然而，在过去的几年里，系统已经开发了热球菌kodakarensis和两个硫化叶菌物种，S。acidocaldarius和S. solfataricus 98/2.在这里，我们描述了外源DNA整合到S的方法的优化。solfataricus PBL 2025、S. solfataricus 98/2衍生物，基于乳糖营养缺陷型，现在允许常规基因失活。
The construction of directed gene deletion mutants is an essential tool in molecular biology that allows functional studies oil the role of genes in their natural environment. For hyperthermophilic archaea, it has been difficult to obtain a reliable system to construct such mutants. However, during the past years, systems have been developed for Thermococcus kodakarensis and two Sulfolobus species, S. acidocaldarius and derivatives of S. solfataricus 98/2. Here we describe an optimization of the method for integration of exogenous DNA into S. solfataricus PBL 2025, an S. solfataricus 98/2 derivative, based on lactose auxotrophy that now allows for routine gene inactivation.