Open reading frame S/L of varicella-zoster virus encodes a cytoplasmic protein expressed in infected cells
Open reading frame S/L of varicella-zoster virus encodes a cytoplasmic protein expressed in infected cells
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DOI：
10.1128/jvi.74.23.11311-11321.2000
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发表时间：
2000-12-01
影响因子：
5.4
通讯作者：
Spaete, RR
中科院分区：
文献类型：
--
作者：
Kemble, GW;Annunziato, P;Spaete, RR
我们报道了在水痘带状疱疹病毒（VZV）基因组中发现的一个新基因，命名为开放阅读框（ORF）S/L。该基因位于原型VZV基因组异构体的左端，在病毒感染的细胞中表达在3 '非翻译区内含有剪接的多腺苷酸化mRNA。序列分析表明，VZV的野生型和减毒株的ORF S/Ls之间存在显著差异。针对ORF S/L的细菌表达部分产生的抗血清与在用VZV临床分离株感染的细胞中合成的21-kDa蛋白质和VZV的原始疫苗株（Oka-ATCC）特异性反应。用其他VZV毒株（包括已在组织培养中广泛传代的野生型毒株和商业生产的Oka疫苗毒株）感染的细胞合成大小在21至30 kDa范围内的蛋白质家族，其与抗ORF S/L抗血清反应。感染重组VZV的MeWO细胞在ORF S/L基因的C-末端区域携带突变，失去了对层和相邻细胞的粘附，导致斑块形态改变。VZV感染细胞的免疫组织化学分析表明ORF S/L蛋白定位于细胞质。ORF S/L蛋白存在于原发性或再活化感染个体的皮肤病变中以及病毒再活化过程中背根神经节的神经元中。
We report the discovery of a novel gene in the varicella-zoster virus (VZV) genome, designated open reading frame (ORF) S/L. This gene, located at the left end of the prototype VZV genome isomer, expresses a polyadenylated mRNA containing a splice within the 3 ' untranslated region in virus-infected cells. Sequence analysis reveals significant differences between the ORF S/Ls of wild-type and attenuated strains of VZV. Antisera raised to a bacterially expressed portion of ORF S/L reacted specifically with a 21-kDa protein synthesized in cells infected with a VZV clinical isolate and with the original vaccine strain of VZV (Oka-ATCC). Cells infected with other VZV strains, including a wild-type strain that has been extensively passaged in tissue culture and commercially produced vaccine strains of Oka, synthesize a family of proteins ranging in size from 21 to 30 kDa that react with the anti-ORF S/L antiserum. MeWO cells infected with recombinant VZV harboring mutations in the C-terminal region of the ORF S/L gene lost adherence to the stratum and adjacent cells, resulting in an altered plaque morphology. Immunohistochemical analysis of VZV-infected cells demonstrated that ORF S/L protein localizes to the cytoplasm. ORF S/L protein was present in skin lesions of individuals with primary or reactivated infection and in the neurons of a dorsal root ganglion during virus reactivation.