Age-dependent expression of the BPI gene in Sutai piglets
Age-dependent expression of the BPI gene in Sutai piglets
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苏泰仔猪中 BPI 基因的年龄依赖性表达
DOI：
10.4238/2012.april.12.1
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发表时间：
2013-01-01
影响因子：
0.4
通讯作者：
Bao, W. B.
中科院分区：
文献类型：
--
作者：
Zhu, J.;Zi, C.;Bao, W. B.
我们通过实时PCR方法比较和分析了新生至断奶后第8、18、30和35天苏泰仔猪BPI基因的表达，以确定其是否参与对ETEC F18引起的疾病的保护。在空肠中18天和35天的表达之间存在显著差异。在十二指肠中，35日龄的表达量与其他发育阶段的表达量也存在显著差异。空肠中的表达在8、18和30天时没有显著差异。因此，猪BPI基因可能是导致断奶仔猪对ETEC F18产生抗性的直接因素，断奶仔猪对ETEC F18的抗性在一定程度上与BPI基因mRNA表达上调有关。
We compared and analyzed expression of the BPI gene of Sutai piglets ranging from newborn to post-weaning days 8, 18, 30, and 35 by the real-time PCR method, in order to determine if it is involved in protection against disease caused by ETEC F18. There was a significant difference between 18- and 35-day expression in the jejunum. There were also significant differences between 35-day expression and expression at the other development stages in the duodenum. There were no significant differences in expression at 8, 18, and 30 days in the jejunum. We conclude that the porcine BPI gene may be the direct factor that caused resistance to ETEC F18 in weaning piglets, and that the resistance to ETEC F18 in weaning piglets is related to upregulation of mRNA expression of the BPI gene to a certain extent.