A microfluidic device integrated with a stretchable microporous membrane controlled by electro-conjugate fluid
A microfluidic device integrated with a stretchable microporous membrane controlled by electro-conjugate fluid
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DOI：
10.1016/j.sna.2023.114332
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发表时间：
2023-03
期刊：
影响因子：
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通讯作者：
Taiki Otomo;T. Matsubara;Kazuhiro Yoshida;Deok‐Ho Kim;M. Ikeuchi;Joon-wan Kim
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文献类型：
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作者：
Taiki Otomo;T. Matsubara;Kazuhiro Yoshida;Deok‐Ho Kim;M. Ikeuchi;Joon-wan Kim
微流控装置可以对细胞施加拉伸刺激来模拟器官的体内环境，作为药物发现筛选中动物试验的替代方法，引起了人们的广泛关注。然而，在之前的论文中报道的这些设备是由大型外部泵和长油管系统组成的笨重的流体动力系统驱动的，这比基于芯片的主设备本身占用的空间要大得多，并且限制了同时处理样品的数量。因此，为了使整个系统小型化，有必要将压力源安装在单个芯片上。在这项研究中，我们建议开发和安装mems制造的微泵，利用电共轭流体（ECF）的强流进入微流控装置来拉伸微孔膜，在微孔膜上可以模拟各种应用的培养细胞。成功制作了该微流控装置，并对其特性进行了实验研究。实验结果表明，该装置可以在与活体环境中器官相似的频率（0.2 Hz）下以应变（5-15%）拉伸微孔膜，证明了使药物发现筛选高效有效的可行性。
Microfluidic devices that can apply stretch stimulation to cells to mimic thein vivoenvironment of organs have attracted significant attention as an alternative to animal testing in drug discovery screening. However, those devices reported in previous papers were driven by bulky fluid power systems composed of large external pumps and long tubing systems, which occupy much larger space than the main chip-based device itself and limit the number of simultaneous sample processing. Therefore, it is necessary to mount pressure sources on a single chip for miniaturizing the entire system. In this study, we propose to develop and mount MEMS-fabricated micropumps utilizing the strong flow of electro-conjugated fluid (ECF) into the microfluidic devices to stretch a microporous membrane, on which cultured cells can be simulated in various applications. The proposed microfluidic device is successfully fabricated, and its characteristics are investigated experimentally. The experimental results show that the device can stretch the microporous membrane with the strain (5–15%) at a frequency (0.2 Hz) similar to the organs’in vivoenvironment, demonstrating the feasibility of making the drug discovery screening efficient and effective.