TEER measurement techniques for in vitro barrier model systems.
TEER measurement techniques for in vitro barrier model systems.
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DOI：
10.1177/2211068214561025
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发表时间：
2015-04
期刊：
影响因子：
--
通讯作者：
Hickman JJ
中科院分区：
文献类型：
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作者：
Srinivasan B;Kolli AR;Esch MB;Abaci HE;Shuler ML;Hickman JJ
跨上皮/跨内皮电阻 (TEER) 是一种广泛接受的定量技术，用于测量内皮和上皮单层细胞培养模型中紧密连接动力学的完整性。在评估药物或化学品的运输之前，TEER 值是细胞屏障完整性的有力指标。 TEER 测量可以实时进行，不会损坏电池，并且通常基于测量欧姆电阻或测量宽频谱范围内的阻抗。各种细胞类型的 TEER 测量已通过市售测量系统以及定制的微流体实现进行报告。利用 TEER 广泛表征的一些屏障模型包括血脑屏障 (BBB)、胃肠道 (GI) 和肺部模型。由于温度、培养基配方和细胞传代次数等因素，TEER 值可能会出现变化。本文的目的是回顾不同的 TEER 测量技术并分析它们的优缺点、TEER 在药物毒性研究中的重要性，检查在一些广泛研究的屏障模型（BBB、胃肠道和肺部）中利用 TEER 测量的各种体外模型和微流控芯片器官实现，并讨论可能影响 TEER 测量的各种因素。
Transepithelial/transendothelial electrical resistance (TEER) is a widely accepted quantitative technique to measure the integrity of tight junction dynamics in cell culture models of endothelial and epithelial monolayers. TEER values are strong indicators of the integrity of the cellular barriers before they are evaluated for transport of drugs or chemicals. TEER measurements can be performed in real-time without cell damage and generally are based on measuring ohmic resistance or measuring impedance across a wide spectrum of frequencies. TEER measurements for various cell types have been reported with commercially available measurement systems and also with custom built microfluidic implementations. Some of the barrier models that have been widely characterized utilizing TEER include the blood-brain barrier (BBB), gastrointestinal (GI) tract, and pulmonary models. Variations in TEER value can arise due to factors such as temperature, medium formulation and passage number of cells. The aim of this paper is to review the different TEER measurement techniques and analyze their strengths and weaknesses, the significance of TEER in drug toxicity studies, examine the various in vitro models and microfluidic organs-on-chips implementations utilizing TEER measurements in some widely studied barrier models (BBB, GI tract and pulmonary), and discuss the various factors that can affect TEER measurements.