The effects of 3,4-diaminopyridine on acetylcholine release at the frog neuromuscular junction.
The effects of 3,4-diaminopyridine on acetylcholine release at the frog neuromuscular junction.
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3,4-二氨基吡啶对青蛙神经肌肉接头乙酰胆碱释放的影响。
DOI：
10.1016/0014-2999(80)90499-9
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发表时间：
1980
影响因子：
5
通讯作者：
I. Marshall
中科院分区：
文献类型：
--
作者：
N. Durant;I. Marshall
本文研究了3，4-二氨基吡啶（3，4-DAP）对蛙缝匠肌自发和诱发递质释放的影响。当浓度为1 mM时，3，4-DAP引起自发巨电位的出现，这种作用在细胞外钙缺乏时也发生。在较低浓度的镁中，Ringer 3，4-DAP（1-10 μM）增加e. p. p.量子含量，但不引起自发巨电位的出现。3，4-DAP延长肌肉动作电位（1 mM），这表明3，4-DAP延长神经末梢动作电位在很大程度上有助于增加诱发的乙酰胆碱释放。3，4-DAP引起巨大自发电位出现的能力被认为与该药物抑制可兴奋膜中钾电导的已知能力无关，并且可能是由于如长春碱所建议的乙酰胆碱的多量子包的释放。因此，3，4-DAP是研究诱发和自发乙酰胆碱释放的有效工具。
The effects of 3,4-diaminopyridine (3,4-DAP) were studied on spontaneous and evoked transmitter release in the frog sartorius muscle preparation. At a concentration of 1 mM, 3,4-DAP caused the appearance of spontaneous giant potentials, this effect of 3,4-DAP also occurred in absence of extracellular calcium. At lower concentrations in magnesium Ringer 3,4-DAP (1–10 μM) increased e.p.p. quantal content without causing the appearance of spontaneous giant potentials. 3,4-DAP prolonged muscle action potentials (1 mM) and it is suggested that prolongation of the nerve terminal action potential by 3,4-DAP contributes largely to the increased evoked acetylcholine release. The ability of 3,4-DAP to cause the appearance of giant spontaneous potentials is considered to be independent of the drug's known ability to inhibit potassium conductance in excitable membranes, and may be due to the release of performed multiquantal packets of acetycholine as suggested for vinblastine. It is concluded that 3,4-DAP is a useful tool in the study of both evoked and spontaneous acetylcholine release.