Insect cardioactive peptides: cardioacceleratory peptide (CAP) activity is blocked in vivo and in vitro with a monoclonal antibody
Insect cardioactive peptides: cardioacceleratory peptide (CAP) activity is blocked in vivo and in vitro with a monoclonal antibody
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昆虫心脏活性肽：用单克隆抗体在体内和体外阻断心脏加速肽 (CAP) 活性
DOI：
10.1523/jneurosci.06-08-02451.1986
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
P. Evans
中科院分区：
文献类型：
--
作者：
N. Tublitz;P. Evans
我们在此证明，特定的单克隆抗体可以用作分析神经肽功能的生理工具。最近从烟草天蛾 Manduca sexta 的中枢神经系统中分离出两种心脏加速肽（CAP）（Tublitz 和 Truman，1985a），并且有人认为它们在成虫羽化和翅膀膨胀期间充当心脏调节神经激素（Tublitz 和 Truman，1985b）。这里提供的证据表明单克隆抗体 6C5 选择性且特异性地沉淀了两种 CAP 的生物活性。体内注射 6C5 显着降低了新生成虫的 CAP 血淋巴滴度。 6C5 治疗还阻断了 CAP 的主要生理效应，即成虫翅膀扩张期间心脏活动的增加。此外，用6C5去除芽后CAP脉冲延长了翅膀膨胀行为的持续时间。因此，通过用 CAP 特异性抗体中和 CAP 血淋巴活性，我们发现 CAP 参与新出现的飞蛾的心脏调节。
We demonstrate here that a specific monoclonal antibody can be utilized as a physiological tool to analyze neuropeptide function. Two cardioacceleratory peptides (CAPs) have been recently isolated from the CNS of the tobacco hawkmoth, Manduca sexta (Tublitz and Truman, 1985a), and it has been suggested that they act as cardioregulatory neurohormones during adult emergence and wing inflation (Tublitz and Truman, 1985b). Evidence is presented here indicating that a monoclonal antibody, 6C5, selectively and specifically precipitated the biological activities of both CAPs. In vivo injections of 6C5 markedly reduced CAP hemolymph titers in newly emerged adults. The 6C5 treatment also blocked the primary physiological effect of the CAPs, the increase in cardiac activity seen during adult wing expansion. In addition, removal of the postemergence CAP pulse with 6C5 prolonged the duration of wing- inflation behavior. Thus, by neutralizing CAP hemolymph activity with a CAP-specific antibody, we have shown that the CAPs are involved in cardioregulation in newly emerged moths.