Genomics reveals historic and contemporary transmission dynamics of a bacterial disease among wildlife and livestock.
Genomics reveals historic and contemporary transmission dynamics of a bacterial disease among wildlife and livestock.
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DOI：
10.1038/ncomms11448
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发表时间：
2016-05-11
影响因子：
16.6
通讯作者：
Cross PC
中科院分区：
文献类型：
--
作者：
Kamath PL;Foster JT;Drees KP;Luikart G;Quance C;Anderson NJ;Clarke PR;Cole EK;Drew ML;Edwards WH;Rhyan JC;Treanor JJ;Wallen RL;White PJ;Robbe-Austerman S;Cross PC
全基因组测序为传染病流行病学提供了基本的见解，但很少用于研究野生动物中细菌病原体的传播动力学。在大黄石生态系统（GYE）中，牛布鲁氏菌病的爆发随着麋鹿血清阳性率的上升而沿着增加。在这里，我们使用基因组的方法来研究布鲁氏菌的进化，跨物种传播和空间传播的GYE。我们发现，布鲁氏菌病被引入到野生动物在这个地区至少有五次。布氏杆菌各谱系的扩散速度不同（每年约3至8公里），而且随着时间的推移而变化。我们还估计了12个主机转换从野牛到麋鹿，和5个从麋鹿到野牛。我们的研究结果支持这样一种观点，即自由放养的麋鹿目前是一个自我维持的布鲁氏菌病水库和牲畜感染的来源，野牛的控制措施不太可能影响附近麋鹿种群中流通的不相关菌株的动态。 野生麋鹿在大黄石地区牛布鲁氏菌病传播和持续中的作用尚不清楚。在这里，Kamath等人分析了来自麋鹿、野牛和牛的245株流产布鲁氏菌分离株的基因组序列，支持麋鹿是家畜感染的重要储存库和来源的观点。
Whole-genome sequencing has provided fundamental insights into infectious disease epidemiology, but has rarely been used for examining transmission dynamics of a bacterial pathogen in wildlife. In the Greater Yellowstone Ecosystem (GYE), outbreaks of brucellosis have increased in cattle along with rising seroprevalence in elk. Here we use a genomic approach to examine Brucella abortus evolution, cross-species transmission and spatial spread in the GYE. We find that brucellosis was introduced into wildlife in this region at least five times. The diffusion rate varies among Brucella lineages (∼3 to 8 km per year) and over time. We also estimate 12 host transitions from bison to elk, and 5 from elk to bison. Our results support the notion that free-ranging elk are currently a self-sustaining brucellosis reservoir and the source of livestock infections, and that control measures in bison are unlikely to affect the dynamics of unrelated strains circulating in nearby elk populations. The role of wild elk in the spread and persistence of bovine brucellosis in the Great Yellowstone area is unclear. Here, Kamath et al. analyse the genomic sequences of 245 Brucella abortus isolates from elk, bison and cattle, supporting the idea that elk is an important reservoir and source of livestock infections.