Microsatellite analysis of a population crash and bottleneck in the Mauna Kea silversword, Argyroxiphium sandwicense ssp sandwicense (Asteraceae), and its implications for reintroduction
Microsatellite analysis of a population crash and bottleneck in the Mauna Kea silversword, Argyroxiphium sandwicense ssp sandwicense (Asteraceae), and its implications for reintroduction
复制标题
DOI：
10.1046/j.1365-294x.2000.01111.x
复制
发表时间：
2000-12-01
影响因子：
4.9
通讯作者：
Robichaux, RH
中科院分区：
文献类型：
--
作者：
Friar, EA;Ladoux, T;Robichaux, RH
毛纳克亚山银剑、毛叶剑蕨（Augyroxiphium sp.）在莫纳克亚山上，外来有蹄类动物数量的增加导致了严重的种群崩溃，而重新引入则导致了种群瓶颈。在本文中，我们使用8个微卫星位点解决这两个人口统计学事件的遗传后果。种群崩溃并不伴随着等位基因或杂合性数量的显著减少。然而，种群瓶颈伴随着观察到的等位基因数量、有效等位基因数量和预期杂合性的显着减少，但观察到的杂合性却没有减少。使用观察到的杂合性和等位基因频率方差计算群体瓶颈的有效大小。这两种方法都证实了人口瓶颈的历史普查规模最多为三个人。结果表明：（i）小种群，即使是由于历史种群规模和范围严重减少而产生的种群，也不一定是遗传上灭绝的;（ii）物种重新引入计划需要仔细构思和执行，并适当考虑重新引入取样的遗传影响。
The Mauna Kea silversword, Augyroxiphium sandwicense ssp. sandwicense, has experienced both a severe population crash associated with an increase in alien ungulate populations on Mauna Kea, and a population bottleneck associated with reintroduction. In this paper, we address the genetic consequences of both demographic events using eight microsatellite loci. The population crash was not accompanied by a significant reduction in number of alleles or heterozygosity. However, the population bottleneck was accompanied by significant reductions in observed number of alleles, effective number of alleles, and expected heterozygosity, though not in observed heterozygosity. The effective size of the population bottleneck was calculated using both observed heterozygosities and allele frequency variances. Both methods corroborated the historical census size of the population bottleneck of at most three individuals. The results suggest that: (i) small populations, even those that result from severe reductions in historical population size and extent, are not necessarily genetically depauperate; and (ii) species reintroduction plans need to be conceived and implemented carefully, with due consideration to the genetic impact of sampling for reintroduction.