Identification and characterization of a myeloid differentiation factor 88 (MyD88) cDNA and gene in Japanese flounder, Paralichthys olivaceus
Identification and characterization of a myeloid differentiation factor 88 (MyD88) cDNA and gene in Japanese flounder, Paralichthys olivaceus
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DOI：
10.1016/j.dci.2005.11.003
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发表时间：
2006-01-01
影响因子：
2.9
通讯作者：
Aoki, Takashi
中科院分区：
文献类型：
--
作者：
Takano, Tomokazu;Kondo, Hidehiro;Aoki, Takashi
白细胞介素-1受体/toll样受体（IL-1 R/TLR）超家族信号转导涉及髓样分化因子88（MyD 88），其作为重要的衔接蛋白。克隆了牙鲆MyD 88（jfMyD 88）的cDNA和基因，其长度分别为1.5和3.01kb。ORF编码285个氨基酸，包含死亡结构域和Toll/IL-1受体结构域。该基因由5个外显子和4个内含子组成。jfMyD 88基因在参与免疫功能的器官中高度表达，包括鳃、肠、肾、皮肤和脾。用迟缓爱德华氏菌感染鱼后三天，用抗jfMyD 88多克隆抗体染色显示肾脏和脾脏中MyD 88阳性细胞的群体增加。这些结果表明MyD 88在比目鱼的先天免疫系统中具有重要作用。(c)\ 2005 Elsevier Ltd.保留所有权利。
The interleukin-1 receptor/toll-like receptor (IL-1R/TLR) superfamily signaling involves myeloid differentiation factor 88 (MyD88) that acts as an important adapter protein. A Japanese flounder (Paralichthys olivaceus) MyD88 (jfMyD88) cDNA and gene were cloned, and found to have lengths of 1.5 and 3.01 kb, respectively. The ORF encodes 285 amino acids that contain a death domain and a Toll/IL-1 receptor domain. The gene is composed of 5 exons and 4 introns. The jfMyD88 gene is highly expressed in organs involved in immune functions, including the gills, intestines, kidney, skin and spleen. Three days after a fish was infected with Edwardsiella tarda, staining with anti-jfMyD88 polyclonal antibody revealed an increased population of MyD88-positive cells in the kidney and spleen. These results imply that MyD88 has an important role in the innate immune system in Japanese flounder. (c)\ 2005 Elsevier Ltd. All rights reserved.