Differential effects of two different routes of immunization on protection against gram-negative sepsis by a detoxified Escherichia coli J5 lipopolysaccharide group B meningococcal outer membrane protein complex vaccine in a burned mouse model.
Differential effects of two different routes of immunization on protection against gram-negative sepsis by a detoxified Escherichia coli J5 lipopolysaccharide group B meningococcal outer membrane protein complex vaccine in a burned mouse model.
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在烧伤小鼠模型中，两种不同的免疫途径对解毒大肠杆菌 J5 脂多糖 B 组脑膜炎球菌外膜蛋白复合物疫苗预防革兰氏阴性败血症的不同效果。
DOI：
10.1097/00004630-200209000-00006
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Cross,AlanS
中科院分区：
文献类型：
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作者：
Neely,AliceN;Bhattacharjee,ApurbaK;Babcock,GeorgeF;Holder,IanA;Cross,AlanS
革兰氏阴性脓毒症会导致烧伤患者的发病率和死亡率。为了确定核心内毒素(脂多糖)两种途径免疫是否能保护烧伤小鼠免于败血症死亡，用核心内毒素疫苗皮下免疫(SC)3次或肌肉注射(IM)1次，然后用核心内毒素疫苗(IP)免疫2次。对照组小鼠用生理盐水或无关抗原免疫。免疫后，小鼠用15%的TBSA火焰烧伤进行免疫抑制，并用肺炎克雷伯氏菌或大肠杆菌攻击焦痂下。疫苗免疫提高了Bothe的存活率。Coli-和K.肺炎小鼠给予SC，但不给予IM，IP。免疫后，总免疫球蛋白滴度高于免疫前滴度，但IM、IP免疫小鼠的滴度高于SC免疫小鼠。同型分析和流式细胞术研究均表明，经IM、IP免疫的小鼠血清比经SC免疫的小鼠血清有更好的调理作用。因此，这种疫苗为烧伤小鼠提供了针对革兰氏阴性脓毒症的特定路线保护；其作用机制并不完全依赖于免疫球蛋白滴度或吞噬功能的增加。
Gram-negative sepsis causes morbidity and mortality in burned patients. To determine whether immunization with core endotoxin (lipopolysaccharide) via one of two routes could protect burned mice from septic death, mice were immunized either three times subcutaneously (SC) or one time intramuscularly (IM) then two times intraperitoneally (IP) with a core-lipopolysaccharide vaccine. Control mice were immunized with either saline or an irrelevant antigen. Postimmunization, mice were immunocompromised with a 15% TBSA flame burn and challenged subeschar withKlebsiella pneumoniaeorEscherichia coli. Vaccine immunization improved the survival of bothE. coli- andK. pneumoniae- challenged mice when given SC but not when given IM, IP. Postimmunization, total immunoglobulin titers were elevated over preimmune titers, but titers in IM, IP-immunized mice were higher than those in SC-immunized mice. Both isotyping and flow cytometry studies indicated that sera from mice immunized via IM, IP opsonized better than sera from mice immunized via SC. Hence, this vaccine provided route-specific protection of burned mice against gram-negative sepsis; its mechanism of action was not solely dependent upon increased immunoglobulin titers or phagocytosis.