Advancing multifocal nonlinear microscopy: development and application of a novel multibeam Yb:KGd(WO4)2 oscillator
Advancing multifocal nonlinear microscopy: development and application of a novel multibeam Yb:KGd(WO4)2 oscillator
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DOI：
10.1364/oe.16.017574
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发表时间：
2008-10-27
期刊：
影响因子：
3.8
通讯作者：
Squier, Jeff A.
中科院分区：
文献类型：
--
作者：
Sheetz, Kraig E.;Hoover, Erich E.;Squier, Jeff A.
我们提出了一种新颖的Yb：KGd（WO 4）（2）振荡器设计，可产生六束时间延迟的253 fs、11 nJ脉冲。这使得多焦点非线性显微镜进行，而不需要复杂的光学复用器。我们展示了我们的设计与12个同时采集的双光子，二次谐波和/或三次谐波图像产生的六个横向分离的焦点。(C)2008年美国光学学会
We present a novel Yb:KGd(WO4)(2) oscillator design that generates six beams of temporally delayed, 253 fs, 11 nJ pulses. This allows multifocal nonlinear microscopy to be performed without the need for complicated optical multiplexers. We demonstrate our design with twelve simultaneously acquired two-photon, second-harmonic and/or third-harmonic images generated from six laterally separated foci. (C) 2008 Optical Society of America