Rokumigan alleviates oxaliplatin‐induced cold hypersensitivity by suppression of transient receptor potential cation channel subfamily M member 8 and subfamily A member 1
Rokumigan alleviates oxaliplatin‐induced cold hypersensitivity by suppression of transient receptor potential cation channel subfamily M member 8 and subfamily A member 1
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Rokumigan 通过抑制瞬时受体电位阳离子通道亚家族 M 成员 8 和亚家族 A 成员 1 减轻奥沙利铂诱导的冷超敏反应
DOI：
10.1002/tkm2.1205
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Yamada Harumi
中科院分区：
文献类型：
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作者：
Shimizu Kanako;Kato Yoshinori;Nakamura Hiroyoshi;Yamada Harumi
AimOxaliplatin(Oxp)是一种用于治疗晚期或复发结直肠癌的化疗药物。OXP在急性治疗阶段引起冷过敏，然而，通过感受低温的瞬时受体势阳离子通道亚家族成员8(TRPM8)和亚家族Ankyrin，成员1(TRPA1)的过度表达。方法采用逆转录-聚合酶链式反应技术，检测了罗枯根(RG)对大鼠4~6腰椎背根神经节冷刺激戒断反应及TRPM8和TRPA1基因表达的影响。RG剂量为1 g/kg/d，连续12 d，Oxp，ip，4 mg/kg，每周2次，连续12天。经Oxp处理后，TRPM8和TRPA1mRNA的过度表达恢复到与RG共处理的赋形剂组相似的水平。结论组成RG的六味中药可能具有减轻Oxp诱导的冷过敏的作用。需要进一步研究分析RG各中草药成分对Trp通道表达的影响。
AimOxaliplatin (Oxp) is a chemotherapeutic agent that is used to treat advanced or recurrent colorectal cancer. Oxp causes cold hypersensitivity in the acute therapy stage, however, by overexpression of the transient receptor potential cation channel subfamily melastatin, member 8 (TRPM8) and subfamily ankyrin, member 1 (TRPA1) ion channels, which sense low temperatures. In this study, we examined the effect of rokumigan (RG) on Oxp‐induced cold hypersensitivity.MethodsWe examined withdrawal response to cold stimulation and analyzed the expression ofTRPM8andTRPA1mRNA in the 4th–6th lumbar vertebrae dorsal root ganglia of rats treated with RG, orally, at 1 g/kg/day for 12 days and Oxp, i.p., at 4 mg/kg twice per week, using quantitative reverse transcription–polymerase chain reaction.ResultsThe withdrawal response to cold stimulation at 4 and 10°C was significantly improved with RG–Oxp co‐treatment. Overexpression ofTRPM8andTRPA1mRNA induced by treatment with Oxp was restored to a level similar to that of the vehicle group by co‐treatment with RG.ConclusionThe six herbs that constitute RG may potentially alleviate Oxp‐induced cold hypersensitivity. Further research is necessary to analyze the effect of each herbal component of RG on the expression of TRP channels.