Effects of domestication on genetic diversity in Chimonanthus praecox: Evidence from chloroplast DNA and amplified fragment length polymorphism data
Effects of domestication on genetic diversity in Chimonanthus praecox: Evidence from chloroplast DNA and amplified fragment length polymorphism data
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DOI：
10.1111/jse.12134
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发表时间：
2015-05-01
影响因子：
3.7
通讯作者：
Fu, Cheng-Xin
中科院分区：
文献类型：
--
作者：
Lu, Yi-Jun;Chen, Chuan;Fu, Cheng-Xin
金银花（L.）林克是中国广泛栽培的特有冬花植物，栽培历史悠久。利用叶绿体DNA （cpDNA）序列和扩增片段长度多态性（AFLP）标记，比较野生和栽培群体的遗传多样性和遗传结构，揭示栽培基因型的地理起源。利用三个组合的叶绿体片段鉴定出9个单倍型。根据叶绿体数据，与栽培组相比，野生组表现出更大的遗传变异和遗传分化，以及更低的基因流。AFLP标记也支持这一趋势。超过40%的cpDNA单倍型在野生种群和栽培种群之间是共享的，共享的单倍型来自多个野生种群，这表明栽培植物有多个起源。主坐标分析、UPGMA分析和AFLP标记结构分析表明，两个野生居群与大部分栽培居群聚集在一起，表明大部分栽培居群主要来源于这两个居群。cpDNA和AFLP联合分析结果表明，现代人工早熟草经历了两个地理起源事件，即中国东部（天目山）和中国西南部（湘桂川黔交界）。
Chimonanthus praecox (L.) Link is a widely cultivated endemic winter-flowering plant in China that has a long cultivation history. Genetic diversity and genetic structure were compared between wild and cultivated groups to reveal the geographic origin of the cultivated genotypes using chloroplast DNA (cpDNA) sequences and amplified fragment length polymorphism (AFLP) markers. Nine haplotypes were identified using three combined chloroplast fragments. Based on chloroplast data, the wild group showed greater genetic variation and genetic differentiation and a lower measure of gene flow compared to the cultivated group. The AFLP markers also supported this trend. More than 40% of the cpDNA haplotypes were shared between wild and cultivated groups, with shared haplotypes originating from multiple wild populations, suggesting multiple origins of cultivated plants. Moreover, principal coordinate analysis, UPGMA, and structure analysis of AFLP markers revealed that two wild populations clustered with most of the cultivated populations of Ch. praecox, suggesting that most of the cultivated populations mainly originated from these two populations. The combined cpDNA and AFLP results indicated that modern cultivated Ch. praecox experienced multiple events of origin involving two geographic origins, eastern China (Tianmu Mountain) and southwestern China (the border of Hunan-Guangxi-Sichuan-Guizhou).