Cross-induction of detoxification genes by environmental xenobiotics and insecticides in the mosquito Aedes aegypti:: Impact on larval tolerance to chemical insecticides
Cross-induction of detoxification genes by environmental xenobiotics and insecticides in the mosquito Aedes aegypti:: Impact on larval tolerance to chemical insecticides
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DOI：
10.1016/j.ibmb.2008.01.004
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发表时间：
2008-05-01
影响因子：
3.8
通讯作者：
David, Jean-Philippe
中科院分区：
文献类型：
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作者：
Poupardin, Rodolphe;Reynaud, Stephane;David, Jean-Philippe
研究了埃及伊蚊幼虫暴露于亚致死剂量拟除虫菊酯类杀虫剂氯菊酯、有机磷替美磷、除草剂莠去津、多环芳烃荧蒽和重金属铜对其后续杀虫剂耐受性、解毒酶活性和解毒基因表达的影响。生物测定显示，接触荧蒽和铜后，幼虫对氯菊酯的耐受性适度增加，而接触莠去津、铜和氯菊酯后，幼虫对替美磷的耐受性适度增加。暴露于氯菊酯、荧蒽和铜的幼虫中细胞色素P450单加氧酶活性被诱导，而谷胱甘肽S-转移酶活性在暴露于荧蒽后被诱导并在暴露于铜后被抑制。使用伊蚊解毒芯片对接触每种异生物质后所有解毒基因的表达模式进行微阵列筛查，识别出由异生物质和杀虫剂诱导的多个基因。使用实时定量 PCR 进行的进一步表达研究证实，杀虫剂和异生素可诱导多种 CYP 基因和一种羧酸酯酶基因。总体而言，这项研究揭示了在受污染的蚊子繁殖地发现的异生物质可能通过特定解毒基因的交叉诱导来影响其对杀虫剂的耐受性。讨论了所涉及的分子机制以及对蚊子控制策略的影响。 (C) 2008 Elsevier Ltd. 保留所有权利。
The effect of exposure of Aedes aegypti larvae to sub-lethal doses of the pyrethroid insecticide permethrin, the organophosphate temephos, the herbicide atrazine, the polycyclic aromatic hydrocarbon fluoranthene and the heavy metal copper on their subsequent tolerance to insecticides, detoxification enzyme activities and expression of detoxification genes was investigated. Bioassays revealed a moderate increase in larval tolerance to permethrin following exposure to fluoranthene and copper while larval tolerance to temephos increased moderately after exposure to atrazine, copper and permethrin. Cytochrome P450 monooxygenases activities were induced in larvae exposed to permethrin, fluoranthene and copper while glutathione S-transferase activities were induced after exposure to fluoranthene and repressed after exposure to copper. Microarray screening of the expression patterns of all detoxification genes following exposure to each xenobiotic with the Aedes Detox Chip identified multiple genes induced by xenobiotics and insecticides. Further expression studies using real-time quantitative PCR confirmed the induction of multiple CYP genes and one carboxylesterase gene by insecticides and xenobiotics. Overall, this study reveals the potential of xenobiotics found in polluted mosquito breeding sites to affect their tolerance to insecticides, possibly through the cross-induction of particular detoxification genes. Molecular mechanisms involved and impact on mosquito control strategies are discussed. (C) 2008 Elsevier Ltd. All rights reserved.