Identification and mapping of QTL for stripe rust resistance in the Chinese wheat cultivar Shumai126
Identification and mapping of QTL for stripe rust resistance in the Chinese wheat cultivar Shumai126
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中国小麦品种舒麦126抗条锈病QTL鉴定与定位
DOI：
10.1094/pdis-09-21-1946-re
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发表时间：
--
期刊：
影响因子：
4.5
通讯作者：
Wu Bihua
中科院分区：
文献类型：
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作者：
Wang Yufan;Hu Yanling;Gong Fangyi;Jin Yarong;Xia Yingjie;He Yu;Jiang Yun;Zhou Qiang;He Jingshu;Feng Lihua;Chen Guoyue;Zheng Youliang;Liu Dengcai;Huang Lin;Wu Bihua
条锈病是由条锈菌引起的。小麦赤霉病是一种危害全球的小麦病害，选育抗病品种是最好的防治策略。中国冬小麦品种蜀麦126(SM126)表现出较强的抗病性。条纹状。白粉菌在田间已有10多年的历史。以太昌29(TC29)与SM126杂交的154份重组自交系为材料，对其抗条锈性的数量性状基因座(QTL)进行了定位。在6个田间环境中，用中国流行小种(中棉所32、中棉所33、中棉所34、中4和HY46)的混合小种对重组自交系进行了检测。条纹状。利用Wheat55K单核苷酸多态(SNP)阵列进行基因分型。在1BL、2AS、2AL、6AS、6BS和7BL染色体上分别定位了6个QTL。所有QTL均由SM126 exceptQYr.sicau-2AL.TheQYr.sicau-1BLandQYr.sicau-2AShad主效应效应，解释的表型变异分别为27.00%~39.91%和11.89%~17.11%，可能分别对应于已知的抗病基因Yr29和Yr69。QYr.sicau-2AL、QYr.sicau-6AS和QYr.sicau-6BS可能是新品种，但影响较小。QYr.sicau-1BL、QYr.sicau-2AS和QYr.sicau-2AL的组合表现出加性效应。将与QYr.sicau-1BL(AX-111056129和AX-108839316)和QYr.sicau-2AS(AX-111557864和AX-110433540)连锁的SNP标记转化为亲本标记，为小麦抗条锈病基因在小麦育种中的应用奠定了基础。
Stripe rust, caused byPuccinia striiformisf. sp.tritici, is a damaging disease of wheat globally, and breeding resistant cultivars is the best control strategy. The Chinese winter wheat cultivar Shumai126 (SM126) exhibited strong resistance toP. striiformisf. sp.triticiin the field for more than 10 years. The objective of this study was to identify and map quantitative trait loci (QTL) for resistance to stripe rust in a population of 154 recombinant inbred lines (RILs) derived from a cross between cultivars Taichang29 (TC29) and SM126. The RILs were tested in six field environments with a mixture of the Chinese prevalent races (CYR32, CYR33, CYR34, Zhong4, and HY46) ofP. striiformisf. sp.triticiand in growth chamber with race CYR34 and genotyped using the Wheat55K single nucleotide polymorphism (SNP) array. Six QTL were mapped on chromosomes 1BL, 2AS, 2AL, 6AS, 6BS, and 7BL, respectively. All QTL were contributed by SM126 exceptQYr.sicau-2AL.TheQYr.sicau-1BLandQYr.sicau-2AShad major effects, explaining 27.00 to 39.91% and 11.89 to 17.11% of phenotypic variances, which may correspond to known resistance genesYr29andYr69, respectively. TheQYr.sicau-2AL,QYr.sicau-6AS, andQYr.sicau-6BSwith minor effects are likely novel.QYr.sicau-7BLwas only detected based on growth chamber seedling data. Additive effects were detected for the combination ofQYr.sicau-1BL,QYr.sicau-2AS, andQYr.sicau-2AL. SNP markers linked toQYr.sicau-1BL(AX-111056129andAX-108839316) andQYr.sicau-2AS(AX-111557864andAX-110433540) were converted to breeder-friendly Kompetitive allele-specific PCR (KASP) markers that would facilitate the deployment of stripe rust resistance genes in wheat breeding.