HUMAN AND BOVINE CORONAVIRUSES RECOGNIZE SIALIC ACID-CONTAINING RECEPTORS SIMILAR TO THOSE OF INFLUENZA C-VIRUSES
HUMAN AND BOVINE CORONAVIRUSES RECOGNIZE SIALIC ACID-CONTAINING RECEPTORS SIMILAR TO THOSE OF INFLUENZA C-VIRUSES
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DOI：
10.1073/pnas.85.12.4526
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发表时间：
1988-06-01
影响因子：
11.1
通讯作者：
PALESE, P
中科院分区：
文献类型：
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作者：
VLASAK, R;LUYTJES, W;PALESE, P
人冠状病毒OC43和牛冠状病毒在37℃孵育下从凝集的鸡红细胞中洗脱，表明存在受体破坏酶。此外，以牛颌下黏液为底物的牛冠状病毒在体外表现出与丙型流感病毒相似的乙酰酯酶活性。此外，用C型流感病毒或牛冠状病毒预处理红细胞可消除冠状病毒或C型流感病毒随后的结合和凝集，而A型流感病毒的结合保持完整。此外，冠状病毒的血凝可以通过用脲源性节杆菌或产气荚膜梭菌神经氨酸酶预处理红细胞或添加含唾液酸的神经节苷类来抑制。这些结果表明，与C型流感病毒一样，人冠状病毒OC43和牛冠状病毒识别o -乙酰化唾液酸或类似衍生物作为细胞受体。
Human coronavirus OC43 and bovine coronavirus elute from agglutinated chicken erythrocytes when incubated at 37.degree.C, suggesting the presence of a receptor-destroying enzyme. Moreover, bovine coronavirus exhibits an acetylesterase activity in vitro using bovine submaxillary mucin as substrate similar to the enzymatic activity found in influenza C viruses. Furthermore, pretreatment of erythrocytes with either influenza C virus or bovine coronavirus eliminates subsequent binding and agglutination by either coronaviruses or influenza C virus, whereas binding of influenza A virus remains intact. In addition, hemagglutination by coronaviruses can be inhibited by pretreatment of erythrocytes with Arthrobacter ureafaciens or Clostridium perfringens neuraminidase or by addition of sialic acid-containing gangliosides. These results suggest that, like influenza C viruses, human coronavirus OC43 and bovine coronavirus recognize O-acetylated sialic acid or a similar derivative as cell receptor.